EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.17.1.9 | CN- | - |
Pseudomonas sp. | |
1.17.1.9 | methanol | - |
Pseudomonas sp. | |
1.17.1.9 | N3- | - |
Pseudomonas sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.17.1.9 | 0.16 | - |
NAD+ | in presence of formate | Pseudomonas sp. | |
1.17.1.9 | 0.62 | - |
formate | in presence of NAD+ | Pseudomonas sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.17.1.9 | 280000 | - |
gel filtration | Pseudomonas sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.17.1.9 | Pseudomonas sp. | - |
101, strain VKM B1545D | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.17.1.9 | - |
Pseudomonas sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.17.1.9 | 2.43 | - |
reaction with NAD+ | Pseudomonas sp. |
1.17.1.9 | 4.55 | - |
reaction with ferricyanide | Pseudomonas sp. |
EC Number | Storage Stability | Organism |
---|---|---|
1.17.1.9 | -20°C, 50% loss of activity ater 1 month | Pseudomonas sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.17.1.9 | 2 ferricyanide + NADH | - |
Pseudomonas sp. | 2 ferrocyanide + NAD+ + H+ | - |
? | |
1.17.1.9 | formate + 2,6-dichloroindophenol | 33% of the activity with NAD+ | Pseudomonas sp. | CO2 + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.17.1.9 | formate + ferricyanide | - |
Pseudomonas sp. | CO2 + ferrocyanide | - |
? | |
1.17.1.9 | formate + NAD+ | - |
Pseudomonas sp. | CO2 + NADH + H+ | - |
? | |
1.17.1.9 | NADH + 2,6-dichloroindophenol | - |
Pseudomonas sp. | NAD+ + reduced 2,6-dichlorophenolindophenol | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.17.1.9 | 40 | - |
- |
Pseudomonas sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.17.1.9 | 50 | - |
1 min, 50% loss of activity | Pseudomonas sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.17.1.9 | 8 | - |
- |
Pseudomonas sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.17.1.9 | NAD+ | - |
Pseudomonas sp. |