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Literature summary extracted from
- NAD-dependent formate dehydrogenase from methylotrophic bacterium, strain 1. Purification and characterization (1979), Eur. J. Biochem., 99, 569-576.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.17.1.9 | CN- | - |
methylotrophic bacterium |  |
| 1.17.1.9 | Dithionitrobenzoate | - |
methylotrophic bacterium | |
| 1.17.1.9 | iodoacetamide | - |
methylotrophic bacterium | |
| 1.17.1.9 | N3- | - |
methylotrophic bacterium | |
| 1.17.1.9 | PCMB | - |
methylotrophic bacterium | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.17.1.9 | 0.11 | - |
NAD+ | - |
methylotrophic bacterium | |
| 1.17.1.9 | 15 | - |
formate | - |
methylotrophic bacterium | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.17.1.9 | 46000 | - |
2 * 46000, SDS-PAGE | methylotrophic bacterium |
| 1.17.1.9 | 70000 | - |
gel filtration | methylotrophic bacterium |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.17.1.9 | methylotrophic bacterium | - |
- |
- |
| 1.17.1.9 | methylotrophic bacterium 1 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.17.1.9 | - |
methylotrophic bacterium |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.17.1.9 | 11 | - |
- |
methylotrophic bacterium |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.17.1.9 | formate + NAD+ | - |
methylotrophic bacterium | CO2 + NADH + H+ | - |
? | |
| 1.17.1.9 | formate + NAD+ | - |
methylotrophic bacterium 1 | CO2 + NADH + H+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.17.1.9 | dimer | 2 * 46000, SDS-PAGE | methylotrophic bacterium |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.17.1.9 | 37 | - |
almost complete inactivation in 2-3 days, -SH compounds protect against inactivation, NAD+ or NADH decreases the inactivation rate, inactivation rate is significantly diminished in presence of formate, strongest stabilization by EDTA | methylotrophic bacterium |
| 1.17.1.9 | 52 | - |
inactivation rate constant is zero | methylotrophic bacterium |
| 1.17.1.9 | 61 | - |
inactivation rate constant: 0.0114/min | methylotrophic bacterium |
| 1.17.1.9 | 65.5 | - |
inactivation rate constant: 0.10/min | methylotrophic bacterium |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.17.1.9 | 5.2 | 10.5 | - |
methylotrophic bacterium |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.17.1.9 | NAD+ | - |
methylotrophic bacterium | |
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