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Literature summary extracted from
- Intermediates and enzymes between alpha-ketoarginine and gamma-guanidinobutyrate in the L-arginine catabolic pathway of Pseudomonas putida (1975), J. Biol. Chem., 250, 5322-5329.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.1.75 | alpha-Hydroxy-delta-guanidino-N-pentanoate | weak | Pseudomonas putida |  |
| 4.1.1.75 | delta-Guanidinopentanoate | - |
Pseudomonas putida | |
| 4.1.1.75 | epsilon-Guanidinohexanoate | - |
Pseudomonas putida | |
| 4.1.1.75 | Ethylguanidine | - |
Pseudomonas putida | |
| 4.1.1.75 | gamma-Guanidinobutanoate | weak | Pseudomonas putida | |
| 4.1.1.75 | gamma-guanidinobutyraldehyde | - |
Pseudomonas putida | |
| 4.1.1.75 | Guanidinoacetate | weak | Pseudomonas putida | |
| 4.1.1.75 | Hexylguanidine | - |
Pseudomonas putida | |
| 4.1.1.75 | L-Arg | weak | Pseudomonas putida | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.2.1.19 | 0.15 | - |
NAD+ | - |
Pseudomonas putida | |
| 1.2.1.19 | 0.26 | - |
4-guanidinobutyraldehyde | - |
Pseudomonas putida | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.2.1.19 | cytoplasm | - |
Pseudomonas putida | 5737 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.1.75 | Ca2+ | activates, half-maximal activity at 0.6 mM | Pseudomonas putida | |
| 4.1.1.75 | Co2+ | activates, half-maximal activity at 0.3 mM | Pseudomonas putida | |
| 4.1.1.75 | Mg2+ | activates, half-maximal activity at 0.3 mM | Pseudomonas putida | |
| 4.1.1.75 | Mn2+ | activates, half-maximal activity at 0.03 mM | Pseudomonas putida | |
| 4.1.1.75 | Ni2+ | activates | Pseudomonas putida | |
| 4.1.1.75 | Zn2+ | activates | Pseudomonas putida | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.2.1.19 | 4-guanidinobutyraldehyde + NAD+ + H2O | Pseudomonas putida | involved in the L-arginine catabolism | 4-guanidinobutyrate + NADH + H+ | - |
? | |
| 1.2.1.19 | 4-guanidinobutyraldehyde + NAD+ + H2O | Pseudomonas putida P2 | involved in the L-arginine catabolism | 4-guanidinobutyrate + NADH + H+ | - |
? | |
| 1.2.1.54 | 4-guanidinobutanal + NAD+ | Pseudomonas putida | arginine catabolic pathway, involved in the degradation of arginine in Pseudomonas putida, cf. EC 1.2.1.19 | 4-guanidinobutanoate + NADH | - |
? | |
| 4.1.1.75 | 5-Guanidino-2-oxopentanoate | Pseudomonas putida | induced by Arg or gamma-guanidinobutyraldehyde | ? | - |
? | |
| 4.1.1.75 | 5-Guanidino-2-oxopentanoate | Pseudomonas putida P2 | induced by Arg or gamma-guanidinobutyraldehyde | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.2.1.19 | Pseudomonas putida | - |
A.T.C.C. 25571 | - |
| 1.2.1.54 | Pseudomonas putida | - |
inducible by L-arginine or its catabolic intermediates | - |
| 4.1.1.75 | Pseudomonas putida | - |
- |
- |
| 4.1.1.75 | Pseudomonas putida P2 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.1.1.75 | - |
Pseudomonas putida |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.2.1.19 | 0.009 | - |
cells grown on succinate and NH3 | Pseudomonas putida |
| 1.2.1.19 | 0.062 | - |
cells grown on L-glutamate | Pseudomonas putida |
| 1.2.1.19 | 0.064 | - |
cells grown on 4-aminobutyric acid | Pseudomonas putida |
| 1.2.1.19 | 0.148 | - |
cells grown on 4-guanidinobutyraldehyde | Pseudomonas putida |
| 1.2.1.19 | 0.156 | - |
cells grown on 4-guanidinobutyric acid | Pseudomonas putida |
| 1.2.1.19 | 0.33 | - |
cells grown on L-arginine | Pseudomonas putida |
| 4.1.1.75 | additional information | - |
- |
Pseudomonas putida |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.2.1.19 | 4-guanidinobutyraldehyde + NAD+ + H2O | - |
Pseudomonas putida | 4-guanidinobutyrate + NADH + H+ | - |
? | |
| 1.2.1.19 | 4-guanidinobutyraldehyde + NAD+ + H2O | involved in the L-arginine catabolism | Pseudomonas putida | 4-guanidinobutyrate + NADH + H+ | - |
? | |
| 1.2.1.19 | 4-guanidinobutyraldehyde + NAD+ + H2O | - |
Pseudomonas putida P2 | 4-guanidinobutyrate + NADH + H+ | - |
? | |
| 1.2.1.19 | 4-guanidinobutyraldehyde + NAD+ + H2O | involved in the L-arginine catabolism | Pseudomonas putida P2 | 4-guanidinobutyrate + NADH + H+ | - |
? | |
| 1.2.1.54 | 4-guanidinobutanal + NAD+ | - |
Pseudomonas putida | 4-guanidinobutanoate + NADH | - |
? | |
| 1.2.1.54 | 4-guanidinobutanal + NAD+ | arginine catabolic pathway, involved in the degradation of arginine in Pseudomonas putida, cf. EC 1.2.1.19 | Pseudomonas putida | 4-guanidinobutanoate + NADH | - |
? | |
| 1.2.1.54 | 4-guanidinobutanal + NAD+ | - |
Pseudomonas putida P2 | 4-guanidinobutanoate + NADH | - |
? | |
| 4.1.1.75 | 5-Guanidino-2-oxopentanoate | i.e. alpha-ketoarginine | Pseudomonas putida | 4-Guanidinobutyraldehyde + CO2 | - |
? | |
| 4.1.1.75 | 5-Guanidino-2-oxopentanoate | i.e. alpha-ketoarginine | Pseudomonas putida P2 | 4-Guanidinobutyraldehyde + CO2 | - |
? | |
| 4.1.1.75 | 5-Guanidino-2-oxopentanoate | induced by Arg or gamma-guanidinobutyraldehyde | Pseudomonas putida | ? | - |
? | |
| 4.1.1.75 | 5-Guanidino-2-oxopentanoate | induced by Arg or gamma-guanidinobutyraldehyde | Pseudomonas putida P2 | ? | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.1.1.75 | 70 | - |
- |
Pseudomonas putida |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.2.1.54 | 80 | - |
10 min, complete inactivation | Pseudomonas putida |
| 4.1.1.75 | 80 | - |
30 min, stable | Pseudomonas putida |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.1.75 | 167 | - |
5-guanidino-2-oxopentanoate | - |
Pseudomonas putida | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.2.1.19 | 8.5 | 9 | - |
Pseudomonas putida |
| 1.2.1.54 | 8.5 | 9 | - |
Pseudomonas putida |
| 4.1.1.75 | 8.5 | - |
- |
Pseudomonas putida |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.2.1.19 | 7 | 10 | - |
Pseudomonas putida |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.2.1.19 | additional information | not NADP+ | Pseudomonas putida | |
| 1.2.1.19 | NAD+ | highly specific for | Pseudomonas putida | |
| 1.2.1.54 | NAD+ | highly specific for | Pseudomonas putida | |
| 4.1.1.75 | NAD+ | required | Pseudomonas putida | |
| 4.1.1.75 | thiamine diphosphate | required | Pseudomonas putida | |
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