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Literature summary extracted from

  • Yorifuji, T.; Koike, K.; Sakurai, T.; Yokoyama, K.
    4-Aminobutyraldehyde and 4-guanidinobutyraldehyde dehydrogenase for arginine degradation in Pseudomonas putida (1986), Agric. Biol. Chem., 50, 2009-2016.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.2.1.19 2-mercaptoethanol and NAD+, no stabilization Pseudomonas putida
1.2.1.54 inactive in absence of 2-mercaptoethanol Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.19 2-Carboxy-2'-hydroxy-5'-sulfoformazylbenzene zincon Pseudomonas putida
1.2.1.19 iodoacetamide
-
 Pseudomonas putida
1.2.1.19 monoiodoacetate slowly Pseudomonas putida
1.2.1.19 N-ethylmaleimide
-
 Pseudomonas putida
1.2.1.19 p-chloromercuribenzoate
-
 Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.19 0.013
-
 Valeraldehyde
-
 Pseudomonas putida
1.2.1.19 0.016
-
 Butyraldehyde
-
 Pseudomonas putida
1.2.1.19 0.13
-
 4-guanidinobutyraldehyde pH 9.5 Pseudomonas putida
1.2.1.19 0.16
-
 4-guanidinobutyraldehyde pH 8.0 Pseudomonas putida
1.2.1.19 0.2
-
 5-Aminovaleraldehyde
-
 Pseudomonas putida
1.2.1.19 0.26
-
 4-Aminobutyraldehyde pH 8.0 Pseudomonas putida
1.2.1.19 0.83
-
 4-Aminobutyraldehyde pH 9.5 Pseudomonas putida
1.2.1.54 0.03
-
 4-guanidinobutyraldehyde
-
 Pseudomonas putida
1.2.1.54 0.14
-
 NAD+
-
 Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.19 57000
-
 4 * 57000, SDS-PAGE Pseudomonas putida
1.2.1.19 83000
-
 gel filtration Pseudomonas putida
1.2.1.19 240000
-
 gel filtration Pseudomonas putida
1.2.1.54 57000
-
 2 * 57000, SDS-PAGE Pseudomonas putida
1.2.1.54 107000
-
 gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.19 4-aminobutanal + NAD+ + H2O Pseudomonas putida involved in the arginine decarboxylase pathway 4-aminobutanoate + NADH + H+
-
 ?
1.2.1.54 4-guanidinobutanal + NAD+ Pseudomonas putida arginine catabolic pathway, involved in the degradation of arginine in Pseudomonas putida, cf. EC 1.2.1.19 4-guanidinobutanoate + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.19 Pseudomonas putida
-
 A.T.C.C. 12633
-
1.2.1.54 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.19 using column chromatography on DEAE-cellulose, DEAE-Toyopearl, Toyopearl HW-55 and Affi-Gel Blue Pseudomonas putida
1.2.1.54
-
 Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.19 additional information
-
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.19 3-aminopropanal + NAD+ + H2O
-
 Pseudomonas putida 3-aminopropanoate + NADH + H+
-
 ?
1.2.1.19 4-aminobutanal + NAD+ + H2O
-
 Pseudomonas putida 4-aminobutanoate + NADH + H+
-
 ?
1.2.1.19 4-aminobutanal + NAD+ + H2O involved in the arginine decarboxylase pathway Pseudomonas putida 4-aminobutanoate + NADH + H+
-
 ?
1.2.1.19 4-guanidinobutyraldehyde + NAD+ + H2O
-
 Pseudomonas putida 4-guanidinobutyrate + NADH + H+
-
 ?
1.2.1.19 4-ureidobutyraldehyde + NAD+
-
 Pseudomonas putida 4-ureidobutyrate + NADH + H+
-
 ?
1.2.1.19 5-aminovaleraldehyde + NAD+ + H2O
-
 Pseudomonas putida 5-aminovalerate + NADH + H+
-
 ?
1.2.1.19 butyraldehyde + NAD+ + H2O
-
 Pseudomonas putida butyrate + NADH + H+
-
 ?
1.2.1.19 propionaldehyde + NAD+ + H2O
-
 Pseudomonas putida propionate + NADH + H+
-
 ?
1.2.1.19 valeraldehyde + NAD+ + H2O
-
 Pseudomonas putida valerate + NADH + H+
-
 ?
1.2.1.54 4-guanidinobutanal + NAD+ specific for Pseudomonas putida 4-guanidinobutanoate + NADH
-
 ?
1.2.1.54 4-guanidinobutanal + NAD+ arginine catabolic pathway, involved in the degradation of arginine in Pseudomonas putida, cf. EC 1.2.1.19 Pseudomonas putida 4-guanidinobutanoate + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.19 tetramer 4 * 57000, SDS-PAGE Pseudomonas putida
1.2.1.54 dimer 2 * 57000, SDS-PAGE Pseudomonas putida

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.19 30
-
 30 min, pH 5.0-8.0, stable Pseudomonas putida
1.2.1.19 30
-
 for 30 min, in 100 mM Na-acetate buffer, pH 5, allmost all activity retained Pseudomonas putida
1.2.1.19 30
-
 for 30 min, in 100 mM K-phosphate buffer, pH 7, allmost all activity retained Pseudomonas putida
1.2.1.19 30
-
 for 30 min, in 100 mM Na-bicine buffer, pH 8, allmost all activity retained Pseudomonas putida
1.2.1.19 60
-
 pH 7.0, 15 min, 35% loss of activity Pseudomonas putida
1.2.1.19 60
-
 for 15 min, in 100 mM K-phosphate buffer, pH 7, 35% of activity lost Pseudomonas putida
1.2.1.54 40
-
 15 min, pH 8.0, 70% loss of activity Pseudomonas putida
1.2.1.54 50
-
 15 min, pH 8.0, 100% loss of activity Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.19 8
-
 optimum toward 4-aminobutyraldehyde Pseudomonas putida
1.2.1.19 8.5 9.5 optimum toward 4-guanidinobutyraldehyde and 5-aminovaleraldehyde Pseudomonas putida
1.2.1.54 9
-
-
 Pseudomonas putida

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.19 6.5 9 4-aminobutyraldehyde, at pH 6.5: about 30% of activity maximum, at pH 9.0: about 70% of activity maximum Pseudomonas putida
1.2.1.19 7 10 4-guanidinobutyraldehyde, at pH 7.0: about 35% of activity maximum, at pH 10.0: about 50% of activity maximum Pseudomonas putida
1.2.1.54 7 10 pH 7.0: about 20% of activity maximum, pH 10.0: about 80% of activity maximum Pseudomonas putida

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.2.1.19 7
-
 most stable at neutral pH Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.19 NAD+ highly specific for Pseudomonas putida
1.2.1.19 NADP+ rate of dehydrogenation about 4% those with NAD+ Pseudomonas putida
1.2.1.54 NAD+ highly specific for Pseudomonas putida