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Literature summary extracted from

  • Hayaishi, O.; Nishizuka, Y.; Tatibana, M.; Takeshita, M.; Kuno, S.
    Enzymatic studies on the metabolism of beta-alanine (1961), J. Biol. Chem., 236, 781-790.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.18 2-mercaptoethanol 0.1 M required for maximal activity Pseudomonas fluorescens
2.6.1.18 pyridoxamine 5'-phosphate shows 20% of pyridoxal 5'-phosphate activation Pseudomonas fluorescens

General Stability

EC Number General Stability Organism
1.2.1.18 dialysis against phosphate buffer, pH 7.0, for 4 h at 4°C results in 80% loss of activity Pseudomonas fluorescens
1.2.1.18 freezing and thawing causes total loss of activity even in the presence of 0.01 M 2-mercaptoethanol Pseudomonas fluorescens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.18 p-chloromercuribenzoate 0.1 mM, 70% inhibition Pseudomonas fluorescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.18 14
-
 beta-Alanine pH 8.0, 35°C, cosubstrate pyruvate Pseudomonas fluorescens
2.6.1.18 62
-
 pyruvate pH 8.0, 35°C, cosubstrate beta-alanine Pseudomonas fluorescens

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.18 Pseudomonas fluorescens
-
-
-
2.6.1.18 Pseudomonas fluorescens
-
 ATCC 11250
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.18
-
 Pseudomonas fluorescens
2.6.1.18 heat treatment, alumina C-gamma gel, protamine sulfate, ammonium sulfate Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.18 additional information
-
-
 Pseudomonas fluorescens
2.6.1.18 56.6
-
-
 Pseudomonas fluorescens

Storage Stability

EC Number Storage Stability Organism
1.2.1.18 2°C, pH 7.0, in presence of 2-mercaptoethanol, stored with least loss of activity Pseudomonas fluorescens
2.6.1.18 -20°C, several weeks, no loss of activity Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.18 3-oxopropanoate + CoA + NAD+
-
 Pseudomonas fluorescens acetyl-CoA + CO2 + NADH
-
 ir
2.6.1.18 beta-alanine + 2-oxobutanoate at 9% of the activity with pyruvate Pseudomonas fluorescens malonic semialdehyde + 2-aminobutanoate
-
 ?
2.6.1.18 beta-alanine + 2-oxohexanoate at 6% of the activity with pyruvate Pseudomonas fluorescens malonic semialdehyde + L-norleucine
-
 ?
2.6.1.18 beta-alanine + 2-oxoisohexanoate at 4% of the activity with pyruvate Pseudomonas fluorescens malonic semialdehyde + L-isoleucine
-
 ?
2.6.1.18 beta-alanine + acetoacetate at 4% of the activity with pyruvate Pseudomonas fluorescens malonic semialdehyde + 3-aminobutanoate
-
 ?
2.6.1.18 beta-alanine + glyoxylic acid at 17% of the activity with pyruvate Pseudomonas fluorescens malonic semialdehyde + glycine
-
 ?
2.6.1.18 beta-alanine + pyruvate i.e. 3-aminopropanoate + 2-oxopropanoate Pseudomonas fluorescens malonic semialdehyde + L-alanine
-
 r
2.6.1.18 pyruvate + 2-aminoisobutanoate at 4% of the activity with beta-alanine Pseudomonas fluorescens L-alanine + 2-oxo-3-methylpropanoate
-
 ?
2.6.1.18 pyruvate + 4-aminobutanoate at 118% of the activity with beta-alanine Pseudomonas fluorescens L-alanine + succinic semialdehyde
-
 ?
2.6.1.18 pyruvate + 5-aminopentanoate at 17% of the activity with beta-alanine Pseudomonas fluorescens L-alanine + 5-oxopentanoate
-
 ?
2.6.1.18 pyruvate + 6-aminohexanoate at 134% of the activity with beta-alanine Pseudomonas fluorescens L-alanine + 6-oxohexanoate
-
 ?
2.6.1.18 pyruvate + DL-3-aminoisobutanoate at 160% of the activity with beta-alanine Pseudomonas fluorescens L-alanine + 3-oxoisobutanoate
-
 ?
2.6.1.18 pyruvate + glycine at 15% of the activity with beta-alanine Pseudomonas fluorescens L-alanine + glyoxylate
-
 ?
2.6.1.18 pyruvate + L-2,4-diaminobutanoate at 4% of the activity with beta-alanine Pseudomonas fluorescens L-alanine + ?
-
 ?
2.6.1.18 pyruvate + L-lysine at 7% of the activity with beta-alanine Pseudomonas fluorescens L-alanine + ?
-
 ?
2.6.1.18 pyruvate + L-ornithine at 1% of the activity with beta-alanine Pseudomonas fluorescens L-alanine + ?
-
 ?
2.6.1.18 pyruvate + taurine at 8% of the activity with beta-alanine Pseudomonas fluorescens L-alanine + 2-oxoethanesulfonic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.6.1.18 beta-alanine-alpha-alanine transaminase
-
 Pseudomonas fluorescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.18 35
-
 assay at Pseudomonas fluorescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.18 5.5 6 activity with NADP+ Pseudomonas fluorescens
1.2.1.18 8.8
-
 activity with NAD+ Pseudomonas fluorescens
2.6.1.18 9.2
-
-
 Pseudomonas fluorescens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.6.1.18 7 11 approx. 35% of maximal activity at pH 7.0, approx. 50% of maximal activity at pH 11.0 Pseudomonas fluorescens

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.18 NAD+ cofactor Pseudomonas fluorescens
1.2.1.18 NADP+ cofactor Pseudomonas fluorescens