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Literature summary extracted from

  • Goodwin, G.W.; Rougraff, P.M.; Davis, E.J.; Harris, R.A.
    Purification and characterization of methylmalonate-semialdehyde dehydrogenase from rat liver. Identity to malonate-semialdehyde dehydrogenase (1989), J. Biol. Chem., 264, 14965-14971.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.18 NADH competitive with NAD+ Rattus norvegicus
1.2.1.27 NADH competitive with NAD+ Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.18 0.0045
-
 3-oxopropanoate
-
 Rattus norvegicus
1.2.1.18 0.0053
-
 2-Methyl-3-oxopropanoate
-
 Rattus norvegicus
1.2.1.18 0.03
-
 CoA
-
 Rattus norvegicus
1.2.1.18 0.15
-
 NAD+
-
 Rattus norvegicus
1.2.1.27 0.0045
-
 malonate semialdehyde
-
 Rattus norvegicus
1.2.1.27 0.0053
-
 2-Methyl-3-oxopropanoate
-
 Rattus norvegicus
1.2.1.27 0.03
-
 CoA
-
 Rattus norvegicus
1.2.1.27 0.15
-
 NAD+
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.1.18 mitochondrion
-
 Rattus norvegicus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.18 58000
-
 4 * 58000, SDS-PAGE Rattus norvegicus
1.2.1.18 250000
-
 gel filtration Rattus norvegicus
1.2.1.27 58000
-
 4 * 58000, SDS-PAGE Rattus norvegicus
1.2.1.27 250000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.27 2-methyl-3-oxopropanoate + CoA + NAD+ Rattus norvegicus
-
 propanoyl-CoA + CO2 + NADH
-
 ir
1.2.1.27 malonate semialdehyde + CoA + NAD+ Rattus norvegicus
-
 acetyl-CoA + CO2 + NADH
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.18 Rattus norvegicus
-
-
-
1.2.1.27 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.18
-
 Rattus norvegicus
1.2.1.27
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.18 liver
-
 Rattus norvegicus
-
1.2.1.27 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.18 1.83
-
-
 Rattus norvegicus
1.2.1.27 1.83
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
1.2.1.27 on ice, 1 mM NAD+, 20 h, 77% activity remains Rattus norvegicus
1.2.1.27 on ice, 10% glycerol, 20 h, 32% activity remains Rattus norvegicus
1.2.1.27 on ice, 20 h, 11% activity remains Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.18 2-methyl-3-oxopropanoate + CoA + NAD+ both stereoisomers Rattus norvegicus propanoyl-CoA + CO2 + NADH
-
 ?
1.2.1.18 3-oxopropanoate + CoA + NAD+
-
 Rattus norvegicus acetyl-CoA + CO2 + NADH
-
 ?
1.2.1.27 2-methyl-3-oxopropanoate + CoA + NAD+
-
 Rattus norvegicus propanoyl-CoA + CO2 + NADH
-
 ir
1.2.1.27 2-methyl-3-oxopropanoate + CoA + NAD+ both stereoisomers are oxidized Rattus norvegicus propanoyl-CoA + CO2 + NADH
-
 ir
1.2.1.27 2-methylbutyraldehyde + CoA + NAD+ low activity Rattus norvegicus ?
-
 ?
1.2.1.27 acetaldehyde + CoA + NAD+ low activity Rattus norvegicus ?
-
 ?
1.2.1.27 butyraldehyde + CoA + NAD+ low activity Rattus norvegicus ?
-
 ?
1.2.1.27 isobutyraldehyde + CoA + NAD+ low activity Rattus norvegicus ?
-
 ?
1.2.1.27 malonate semialdehyde + CoA + NAD+
-
 Rattus norvegicus acetyl-CoA + CO2 + NADH
-
 ir
1.2.1.27 malondialdehyde + CoA + NAD+ low activity Rattus norvegicus ?
-
 ?
1.2.1.27 propanal + NAD+
-
 Rattus norvegicus ? + NADH
-
 ?
1.2.1.27 succinate semialdehyde + CoA + NAD+ very low activity Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.18 tetramer 4 * 58000, SDS-PAGE Rattus norvegicus
1.2.1.27 tetramer 4 * 58000, SDS-PAGE Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.18 8
-
 reaction with 2-methyl-3-oxopropanoate Rattus norvegicus
1.2.1.27 8
-
 2-methyl-3-oxopropanoate + CoA + NAD+ Rattus norvegicus
1.2.1.27 9
-
 propanal + NAD+ Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.27 7 8.5 methylmalonate semialdehyde oxidation, less than 90% of maximal activity above and below Rattus norvegicus
1.2.1.27 8 9.5 propanal oxidation, less than 50% of maximal activity above and below Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.18 NAD+ cofactor Rattus norvegicus
1.2.1.27 CoA
-
 Rattus norvegicus
1.2.1.27 NAD+
-
 Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.2.1.27 0.0031
-
 NADH
-
 Rattus norvegicus