EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.2.3.13 | 2-mercaptoethanol | - |
Arthrobacter globiformis | |
1.2.3.13 | cysteine | - |
Arthrobacter globiformis | |
1.2.3.13 | dithiothreitol | - |
Arthrobacter globiformis | |
1.2.3.13 | GSH | optimal activity at 0.002 mM, can be replaced by 2-mercaptoethanol, cysteine or dithiothreitol | Arthrobacter globiformis |
EC Number | General Stability | Organism |
---|---|---|
1.2.3.13 | stabilizing effects of activity with ammonium sulfate, NaCl, Na2SO4 or MgSO4 | Arthrobacter globiformis |
1.13.11.15 | dialysis against buffer for several hours at 0°C, no appreciable loss of activity | Arthrobacter sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.2.3.13 | EDTA | potent inhibitor, other metal chelators ineffective | Arthrobacter globiformis | |
1.14.14.9 | EDTA | inhibits non-purified enzyme, ammonium sulfate precipitate | Arthrobacter sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.2.3.13 | Mg2+ | optimal activity at 0.01 mM | Arthrobacter globiformis | |
1.2.3.13 | Mn2+ | optimal activity at 0.01 mM | Arthrobacter globiformis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.16 | succinate semialdehyde + NADP+ + H2O | Arthrobacter sp. | degradation of 3,4-dihydroxyphenylacetate in Arthrobacter sp. | succinate + NADPH + H+ | - |
? | |
1.2.1.16 | succinate semialdehyde + NADP+ + H2O | Arthrobacter sp. PRL W15 | degradation of 3,4-dihydroxyphenylacetate in Arthrobacter sp. | succinate + NADPH + H+ | - |
? | |
1.2.1.60 | 5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+ | Arthrobacter globiformis | involved in the meta-cleavage pathway of 4-hydroxyphenylacetate | 5-carboxymethyl-2-hydroxymuconate + NADH | - |
? | |
1.2.1.60 | 5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+ | Arthrobacter globiformis PRL W15 | involved in the meta-cleavage pathway of 4-hydroxyphenylacetate | 5-carboxymethyl-2-hydroxymuconate + NADH | - |
? | |
1.2.3.13 | 4-hydroxyphenylpyruvate + O2 | Arthrobacter globiformis | - |
4-hydroxyphenylacetate + CO2 | - |
? | |
1.2.3.13 | 4-hydroxyphenylpyruvate + O2 | Arthrobacter globiformis PRL W15 | - |
4-hydroxyphenylacetate + CO2 | - |
? | |
1.13.11.15 | 3,4-dihydroxyphenylacetate + O2 | Arthrobacter sp. | enzyme is involved in catabolism of L-Tyr | 2-hydroxy-5-carboxymethylmuconate semialdehyde | - |
? | |
1.14.14.9 | 4-hydroxyphenylacetate + NADH + O2 | Arthrobacter sp. | degradation of tyrosine with 3,4-dihydroxyphenylacetate as key intermediate | 3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
? | |
1.14.14.9 | 4-hydroxyphenylacetate + NADH + O2 | Arthrobacter sp. PRL W15 | degradation of tyrosine with 3,4-dihydroxyphenylacetate as key intermediate | 3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.1.16 | Arthrobacter sp. | - |
- |
- |
1.2.1.60 | Arthrobacter globiformis | - |
- |
- |
1.2.1.60 | Arthrobacter globiformis PRL W15 | - |
- |
- |
1.2.3.13 | Arthrobacter globiformis | - |
- |
- |
1.13.11.15 | Arthrobacter sp. | - |
- |
- |
1.14.14.9 | Arthrobacter sp. | - |
PRL W15 | - |
1.14.14.9 | Arthrobacter sp. PRL W15 | - |
PRL W15 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.14.9 | ultracentrifugation, ammonium sulfate precipitation | Arthrobacter sp. |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.2.1.60 | cell culture | - |
Arthrobacter globiformis | - |
1.2.3.13 | culture condition:L-tyrosine-grown cell | - |
Arthrobacter globiformis | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.2.1.16 | additional information | - |
- |
Arthrobacter sp. |
1.14.14.9 | additional information | - |
activity is reduced by about 40% if 3-hydroxyphenylacetate is used as substrate instead of 4-hydroxyphenylacetate | Arthrobacter sp. |
EC Number | Storage Stability | Organism |
---|---|---|
1.2.3.13 | -20°C, ammonium sulfate precipitation or crude extract, 4 weeks | Arthrobacter globiformis |
1.13.11.15 | 10°C, lyophilized preparations of the enzyme, 8 months, stable | Arthrobacter sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.16 | succinate semialdehyde + NADP+ + H2O | - |
Arthrobacter sp. | succinate + NADPH + H+ | - |
? | |
1.2.1.16 | succinate semialdehyde + NADP+ + H2O | degradation of 3,4-dihydroxyphenylacetate in Arthrobacter sp. | Arthrobacter sp. | succinate + NADPH + H+ | - |
? | |
1.2.1.16 | succinate semialdehyde + NADP+ + H2O | - |
Arthrobacter sp. PRL W15 | succinate + NADPH + H+ | - |
? | |
1.2.1.16 | succinate semialdehyde + NADP+ + H2O | degradation of 3,4-dihydroxyphenylacetate in Arthrobacter sp. | Arthrobacter sp. PRL W15 | succinate + NADPH + H+ | - |
? | |
1.2.1.60 | 5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+ | involved in the meta-cleavage pathway of 4-hydroxyphenylacetate | Arthrobacter globiformis | 5-carboxymethyl-2-hydroxymuconate + NADH | - |
? | |
1.2.1.60 | 5-carboxymethyl-2-hydroxymuconate semialdehyde + H2O + NAD+ | involved in the meta-cleavage pathway of 4-hydroxyphenylacetate | Arthrobacter globiformis PRL W15 | 5-carboxymethyl-2-hydroxymuconate + NADH | - |
? | |
1.2.3.13 | 4-hydroxyphenylpyruvate + O2 | - |
Arthrobacter globiformis | 4-hydroxyphenylacetate + CO2 | - |
? | |
1.2.3.13 | 4-hydroxyphenylpyruvate + O2 | - |
Arthrobacter globiformis PRL W15 | 4-hydroxyphenylacetate + CO2 | - |
? | |
1.2.3.13 | glyoxylate + O2 | 19% of the activity compared to 4-hydroxyphenylpyruvate as substrate | Arthrobacter globiformis | CO2 + CO2 | - |
? | |
1.2.3.13 | glyoxylate + O2 | 19% of the activity compared to 4-hydroxyphenylpyruvate as substrate | Arthrobacter globiformis PRL W15 | CO2 + CO2 | - |
? | |
1.2.3.13 | phenylpyruvate + O2 | 84% of the activity compared to 4-hydroxyphenylpyruvate as substrate | Arthrobacter globiformis | phenylacetate + CO2 | - |
? | |
1.2.3.13 | phenylpyruvate + O2 | 84% of the activity compared to 4-hydroxyphenylpyruvate as substrate | Arthrobacter globiformis PRL W15 | phenylacetate + CO2 | - |
? | |
1.13.11.15 | 3,4-dihydroxyphenylacetate + O2 | - |
Arthrobacter sp. | 2-hydroxy-5-carboxymethylmuconate semialdehyde | - |
? | |
1.13.11.15 | 3,4-dihydroxyphenylacetate + O2 | enzyme is involved in catabolism of L-Tyr | Arthrobacter sp. | 2-hydroxy-5-carboxymethylmuconate semialdehyde | - |
? | |
1.14.14.9 | 3-hydroxyphenylacetate + NAD(P)H + O2 | - |
Arthrobacter sp. | 3,4-hydroxyphenylacetate + NAD(P)+ + H2O | - |
? | |
1.14.14.9 | 3-hydroxyphenylacetate + NAD(P)H + O2 | - |
Arthrobacter sp. PRL W15 | 3,4-hydroxyphenylacetate + NAD(P)+ + H2O | - |
? | |
1.14.14.9 | 4-hydroxyphenylacetate + NAD(P)H + O2 | enzyme is induced by 4-hydroxyphenylacetate, no constitutive synthesis | Arthrobacter sp. | 3,4-dihydroxyphenylacetate + NAD(P)+ + H2O | - |
ir | |
1.14.14.9 | 4-hydroxyphenylacetate + NAD(P)H + O2 | enzyme is induced by 4-hydroxyphenylacetate, no constitutive synthesis | Arthrobacter sp. PRL W15 | 3,4-dihydroxyphenylacetate + NAD(P)+ + H2O | - |
ir | |
1.14.14.9 | 4-hydroxyphenylacetate + NADH + O2 | degradation of tyrosine with 3,4-dihydroxyphenylacetate as key intermediate | Arthrobacter sp. | 3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
? | |
1.14.14.9 | 4-hydroxyphenylacetate + NADH + O2 | degradation of tyrosine with 3,4-dihydroxyphenylacetate as key intermediate | Arthrobacter sp. PRL W15 | 3,4-dihydroxyphenylacetate + NAD+ + H2O | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.14.9 | 28 | - |
assay at | Arthrobacter sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.2.3.13 | - |
- |
10% loss of activity per h | Arthrobacter globiformis |
1.13.11.15 | 55 | - |
5 min, stable | Arthrobacter sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.2.3.13 | 7.5 | - |
- |
Arthrobacter globiformis |
1.14.14.9 | 7.5 | - |
assay at | Arthrobacter sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.16 | NAD+ | rate with NADP+ is about twice that with NAD+, crude extract | Arthrobacter sp. | |
1.2.1.16 | NADP+ | rate with NADP+ is about twice that with NAD+, crude extract | Arthrobacter sp. | |
1.2.1.60 | NAD+ | can not be replaced by NADP+ | Arthrobacter globiformis | |
1.2.3.13 | FAD | optimal activity at 10 nM, can not be replaced by NAD+, NADP+ or FMN | Arthrobacter globiformis | |
1.2.3.13 | thiamine diphosphate | optimal activity at 0.004 mM, absolute requirement | Arthrobacter globiformis | |
1.14.14.9 | additional information | similar enzyme which oxidizes both NADH and NADPH | Arthrobacter sp. |