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Literature summary extracted from

  • Zeikus, J.G.; Fuchs, G.; Kenealy, W.; Thauer, R.K.
    Oxidoreductases involved in cell carbon synthesis of Methanobacterium thermoautotrophicum (1977), J. Bacteriol., 132, 604-613.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.13 0.01
-
 NADPH
-
 Methanothermobacter thermautotrophicus
1.2.1.13 0.1
-
 NADH
-
 Methanothermobacter thermautotrophicus
1.2.1.13 0.1
-
 NADP+
-
 Methanothermobacter thermautotrophicus
1.2.1.13 0.2
-
 NAD+
-
 Methanothermobacter thermautotrophicus
1.2.1.13 15
-
 phosphate
-
 Methanothermobacter thermautotrophicus
1.2.1.13 30
-
 arsenate
-
 Methanothermobacter thermautotrophicus

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.13 Methanothermobacter thermautotrophicus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.13 D-glyceraldehyde 3-phosphate + arsenate + NADP+
-
 Methanothermobacter thermautotrophicus 3-phospho-D-glyceroyl arsenate + NADPH
-
 ?
1.2.1.13 D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Methanothermobacter thermautotrophicus 3-phospho-D-glyceroyl phosphate + NADH
-
 ?
1.2.1.13 D-glyceraldehyde 3-phosphate + phosphate + NADP+
-
 Methanothermobacter thermautotrophicus 3-phospho-D-glyceroyl phosphate + NADPH
-
 r

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.13 60
-
 inactivation above Methanothermobacter thermautotrophicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.13 8.4
-
-
 Methanothermobacter thermautotrophicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.13 NAD+ cofactor Methanothermobacter thermautotrophicus
1.2.1.13 NAD+ activity with NADP+ is higher than with NAD+ Methanothermobacter thermautotrophicus
1.2.1.13 NADP+ cofactor Methanothermobacter thermautotrophicus