Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ferri, G.; Comerio, G.; Iadarola, P.; Zapponi, M.C.; Speranza, M.L.
    Subunit structure and activity of glyceraldehyde-3-phosphate dehydrogenase from spinach chloroplasts (1978), Biochim. Biophys. Acta, 522, 19-31.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.13 iodoacetate preincubation with glyceraldehyde-phosphate, 10fold molar excess over iodoacetate, prevents inactivation Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.13 additional information
-
 additional information
-
 Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.1.13 chloroplast
-
 Spinacia oleracea 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.13 37000
-
 x * 43000 + x * 37000, SDS-PAGE Spinacia oleracea
1.2.1.13 43000
-
 x * 43000 + x * 37000, SDS-PAGE Spinacia oleracea

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.13 Spinacia oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.13
-
 Spinacia oleracea

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.13 13.92
-
 NAD+-dependent activity Spinacia oleracea
1.2.1.13 15.4
-
 NADP+-dependent activity Spinacia oleracea

Storage Stability

EC Number Storage Stability Organism
1.2.1.13 4°C, pH 8.5, protein concentration 2.5 mg/ml, apoprotein, complete loss of activity after 48 h Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.13 D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Spinacia oleracea 3-phospho-D-glyceroyl phosphate + NADH
-
 r
1.2.1.13 D-glyceraldehyde 3-phosphate + phosphate + NADP+
-
 Spinacia oleracea 3-phospho-D-glyceroyl phosphate + NADPH
-
 r

Subunits

EC Number Subunits Comment Organism
1.2.1.13 ? x * 43000 + x * 37000, SDS-PAGE Spinacia oleracea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.13 8 8.8 both directions Spinacia oleracea

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.13 NADP+ cofactor Spinacia oleracea