EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.12 | 5 | - |
NAD+ | pH 8.5, 65°C | Saccharolobus solfataricus | |
1.2.1.13 | 0.01 | - |
NADPH | - |
Saccharolobus solfataricus | |
1.2.1.13 | 0.02 | - |
NADP+ | - |
Saccharolobus solfataricus | |
1.2.1.13 | 0.1 | - |
NADP+ | - |
Saccharolobus solfataricus | |
1.2.1.13 | 0.18 | - |
NADH | - |
Saccharolobus solfataricus | |
1.2.1.13 | 5 | - |
NAD+ | - |
Saccharolobus solfataricus | |
1.2.1.13 | 88 | - |
arsenate | - |
Saccharolobus solfataricus | |
1.2.1.13 | 182 | - |
phosphate | - |
Saccharolobus solfataricus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.2.1.12 | 41000 | - |
4 * 41000, SDS-PAGE | Saccharolobus solfataricus |
1.2.1.12 | 155000 | - |
gel filtration | Saccharolobus solfataricus |
1.2.1.13 | 41000 | - |
4 * 41000, SDS-PAGE | Saccharolobus solfataricus |
1.2.1.13 | 155000 | - |
gel filtration | Saccharolobus solfataricus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.12 | D-glyceraldehyde 3-phosphate + phosphate + NAD+ | Saccharolobus solfataricus | - |
3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.1.12 | Saccharolobus solfataricus | P39460 | - |
- |
1.2.1.13 | Saccharolobus solfataricus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.1.12 | - |
Saccharolobus solfataricus |
1.2.1.13 | - |
Saccharolobus solfataricus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.2.1.12 | 9.2 | - |
pH 8.5, 65°C | Saccharolobus solfataricus |
1.2.1.13 | 9.2 | - |
- |
Saccharolobus solfataricus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.12 | D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Saccharolobus solfataricus | 3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? | |
1.2.1.12 | D-glyceraldehyde 3-phosphate + phosphate + NAD+ | the enzyme utilizes either NAD+ or NADP+ as coenzyme but its affinity for the latter is about 50fold higher | Saccharolobus solfataricus | 3-phospho-D-glyceroyl phosphate + NADH + H+ | - |
? | |
1.2.1.13 | D-glyceraldehyde 3-phosphate + arsenate + NADP+ | - |
Saccharolobus solfataricus | 3-phospho-D-glyceroyl arsenate + NADPH | - |
? | |
1.2.1.13 | D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Saccharolobus solfataricus | 3-phospho-D-glyceroyl phosphate + NADH | - |
r | |
1.2.1.13 | D-glyceraldehyde 3-phosphate + phosphate + NADP+ | - |
Saccharolobus solfataricus | 3-phospho-D-glyceroyl phosphate + NADPH | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.2.1.12 | tetramer | 4 * 41000, SDS-PAGE | Saccharolobus solfataricus |
1.2.1.13 | tetramer | 4 * 41000, SDS-PAGE | Saccharolobus solfataricus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.2.1.12 | 87 | - |
- |
Saccharolobus solfataricus |
1.2.1.13 | 87 | - |
- |
Saccharolobus solfataricus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.2.1.12 | 87 | - |
activity decreases rapidly above 87°C | Saccharolobus solfataricus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.2.1.12 | 87 | - |
half-life: 45 min | Saccharolobus solfataricus |
1.2.1.12 | 95 | - |
complete inactivation after 30 min | Saccharolobus solfataricus |
1.2.1.13 | 70 | - |
40 min, stable | Saccharolobus solfataricus |
1.2.1.13 | 87 | - |
half-life: 45 min | Saccharolobus solfataricus |
1.2.1.13 | 95 | - |
30 min, complete inactivation | Saccharolobus solfataricus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.12 | 20 | - |
NAD+ | pH 8.5, 65°C | Saccharolobus solfataricus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.2.1.12 | 8.5 | - |
- |
Saccharolobus solfataricus |
1.2.1.13 | 8.5 | - |
both directions | Saccharolobus solfataricus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.12 | NAD+ | the enzyme utilizes either NAD+ or NADP+ as coenzyme but its affinity for NADP+ is about 50fold higher | Saccharolobus solfataricus | |
1.2.1.13 | NAD+ | activity with NADP+ is about 50fold higher than that determined for NAD+ | Saccharolobus solfataricus | |
1.2.1.13 | NADP+ | cofactor | Saccharolobus solfataricus |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
1.2.1.12 | Saccharolobus solfataricus | calculated from sequence | - |
8.7 |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.12 | 0.25 | - |
NAD+ | pH 8.5, 65°C | Saccharolobus solfataricus |