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Literature summary extracted from

  • Suzuki, K.; Imahori, K.
    Isolation and some properties of glyceraldehyde 3-phosphate dehydrogenase from vegetative cells of Bacillus cereus (1973), J. Biochem., 73, 97-106.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.12 additional information enzyme contains 8 SH groups per tetramer of which 4 are highly reactive and essential for activity Bacillus cereus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.12 Cu2+
-
 Bacillus cereus
1.2.1.12 iodoacetate
-
 Bacillus cereus
1.2.1.12 Na2S4O6
-
 Bacillus cereus
1.2.1.12 NEM
-
 Bacillus cereus
1.2.1.12 Sodium tetrathionate
-
 Bacillus cereus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.12 0.27
-
 NAD+
-
 Bacillus cereus
1.2.1.12 0.51
-
 D-glyceraldehyde 3-phosphate
-
 Bacillus cereus
1.2.1.12 1
-
 arsenate
-
 Bacillus cereus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.12 additional information
-
 high content of beta-structure, 45% Bacillus cereus
1.2.1.12 36000
-
 4 * 36000, SDS-PAGE Bacillus cereus

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.12 Bacillus cereus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.12
-
 Bacillus cereus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.12 vegetative
-
 Bacillus cereus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.12 additional information
-
-
 Bacillus cereus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.12 D-glyceraldehyde 3-phosphate + arsenate + NAD+
-
 Bacillus cereus 3-phospho-D-glyceroyl arsenate + NADH + H+
-
 r
1.2.1.12 D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Bacillus cereus 3-phospho-D-glyceroyl phosphate + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.12 tetramer 4 * 36000, SDS-PAGE Bacillus cereus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.12 50
-
 pH 7.5, 60 min, stable Bacillus cereus
1.2.1.12 60
-
 pH 7.5, 30 min, about 40% loss of activity Bacillus cereus
1.2.1.12 70
-
 loss of activity within a few min Bacillus cereus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.12 8.6
-
-
 Bacillus cereus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.12 7 9.5 pH 7.0: about 70% of maximal activity, pH 9.5: about 55% of maximal activity Bacillus cereus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.12 NAD+
-
 Bacillus cereus