EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.12 | 0.1 | - |
NAD+ | - |
Saccharomyces cerevisiae | |
1.2.1.12 | 0.6 | - |
D-glyceraldehyde 3-phosphate | - |
Saccharomyces cerevisiae | |
1.2.1.12 | 1.5 | - |
phosphate | - |
Saccharomyces cerevisiae |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.2.1.12 | 35000 | - |
4 * 35000, SDS-PAGE | Saccharomyces cerevisiae |
1.2.1.12 | 145000 | - |
gel filtration | Saccharomyces cerevisiae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.1.12 | Saccharomyces cerevisiae | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.1.12 | - |
Saccharomyces cerevisiae |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.2.1.12 | additional information | - |
- |
Saccharomyces cerevisiae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.12 | 3-phospho-D-glyceroyl phosphate + NADH | - |
Saccharomyces cerevisiae | D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
r | |
1.2.1.12 | D-glyceraldehyde 3-phosphate + 3-acetylpyridine NAD+ + phosphate | - |
Saccharomyces cerevisiae | 3-phospho-D-glyceroyl phosphate + ? | - |
? | |
1.2.1.12 | D-glyceraldehyde 3-phosphate + arsenate + NAD+ | - |
Saccharomyces cerevisiae | 3-phospho-D-glyceroyl arsenate + NADH | - |
ir | |
1.2.1.12 | D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Saccharomyces cerevisiae | 3-phospho-D-glyceroyl phosphate + NADH | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.2.1.12 | tetramer | 4 * 35000, SDS-PAGE | Saccharomyces cerevisiae |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.2.1.12 | 27 | - |
pH 8.5, half-life: 2 days | Saccharomyces cerevisiae |
1.2.1.12 | 50 | - |
pH 7.5, half-life: 3 h | Saccharomyces cerevisiae |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.12 | 16.7 | - |
D-glyceraldehyde 3-phosphate | - |
Saccharomyces cerevisiae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.2.1.12 | 8.5 | - |
phosphorolysis and arsenolysis | Saccharomyces cerevisiae |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.2.1.12 | 4.5 | 11 | rapid inactivation below pH 4.5 and above pH 11.0 | Saccharomyces cerevisiae |
1.2.1.12 | 8.5 | - |
27°C, half-life: 2 days | Saccharomyces cerevisiae |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.12 | 3-acetylpyridine-NAD+ | - |
Saccharomyces cerevisiae | |
1.2.1.12 | NAD+ | weak binding of NAD+ to the enzyme | Saccharomyces cerevisiae | |
1.2.1.12 | NADH | cofactor | Saccharomyces cerevisiae |