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Literature summary extracted from

  • Maurer, K.H.; Pfeiffer, F.; Zehender, H.; Mecke, D.
    Characterization of two glyceraldehyde-3-phosphate dehydrogenase isoenzymes from the pentalenolactone producer Streptomyces arenae (1983), J. Bacteriol., 153, 930-936.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.12 pentalenolactone pentalenolactone-sensitive enzyme is strongly inhibited, pentalenolactone-insensitive enzyme is not inhibited Streptomyces arenae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.12 0.1
-
 D-glyceraldehyde 3-phosphate pentalenolactone-insensitive enzyme Streptomyces arenae
1.2.1.12 0.11
-
 NAD+ pentalenolactone-sensitive enzyme Streptomyces arenae
1.2.1.12 0.12
-
 NAD+ pentalenolactone-insensitive enzyme Streptomyces arenae
1.2.1.12 0.25
-
 D-glyceraldehyde 3-phosphate pentalenolactone-sensitive enzyme Streptomyces arenae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.12 43000
-
 4 * 43000, pentalenolactone-sensitive enzyme, gel filtration Streptomyces arenae
1.2.1.12 43000
-
 8 * 43000, pentalenolactone-insensitive enzyme, gel filtration Streptomyces arenae
1.2.1.12 180000
-
 gel filtration, pentalenolactone-sensitive enzyme Streptomyces arenae
1.2.1.12 290000
-
 gel filtration, pentalenolactone-insensitive enzyme Streptomyces arenae

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.12 Streptomyces arenae
-
 TÜ469, contains pentalenolactone-insensitive glyceraldehyde-3-phosphate dehydrogenase under conditions of production of pentalenolactone. In complex medium no pentalenolactone production is observed and a pentalenolactone-sensitive D-glyceraldehyde 3-phosphate dehydrogenase, rather than the insensitive enzyme can be detected
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.12
-
 Streptomyces arenae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.12 33
-
 pentalenolactone-insensitive enzyme Streptomyces arenae
1.2.1.12 112
-
 pentalenolactone-sensitive enzyme Streptomyces arenae

Storage Stability

EC Number Storage Stability Organism
1.2.1.12 4°C, pentalenolactone-insensitive enzyme stable up to 6 months. Pentalenolactone-sensitive enzyme loses activity with a half-life of 7 days Streptomyces arenae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.12 3-phospho-D-glyceroyl phosphate + NADH
-
 Streptomyces arenae D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 r
1.2.1.12 D-glyceraldehyde 3-phosphate + arsenate + NAD+
-
 Streptomyces arenae 3-phospho-D-glyceroyl arsenate + NADH
-
 ?
1.2.1.12 D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Streptomyces arenae 3-phospho-D-glyceroyl phosphate + NADH
-
 r

Subunits

EC Number Subunits Comment Organism
1.2.1.12 octamer 8 * 43000, pentalenolactone-insensitive enzyme, gel filtration Streptomyces arenae
1.2.1.12 tetramer 4 * 43000, pentalenolactone-sensitive enzyme, gel filtration Streptomyces arenae

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.12 NAD+
-
 Streptomyces arenae
1.2.1.12 NADH cofactor Streptomyces arenae