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Literature summary extracted from

  • Krishnan, G.; Altekar, W.
    Characterization of a halophilic glyceraldehyde-3-phosphate dehydrogenase from the archaebacterium Haloarcula vallismortis (1990), J. Gen. Appl. Microbiol., 36, 19-32.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.12 2-mercaptoethanol maximal activation at 5 mM, dependent on sulfhydryl compound Haloarcula vallismortis
1.2.1.12 GSH dependent on sulfhydryl compound Haloarcula vallismortis

General Stability

EC Number General Stability Organism
1.2.1.12 very stable in 4 M KCl. Inactivated within 12 h in 0.5 M KCl, 50 mM Tris-HCl, pH 8.0, at 25°C Haloarcula vallismortis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.12 (NH4)2SO4
-
 Haloarcula vallismortis
1.2.1.12 Cu2+ 1 mM CuCl2, complete inhibition Haloarcula vallismortis
1.2.1.12 HgCl2 1 mM, complete inhibition Haloarcula vallismortis
1.2.1.12 iodoacetamide 5 mM, complete inhibition Haloarcula vallismortis
1.2.1.12 PCMB 5 mM, complete inhibition Haloarcula vallismortis
1.2.1.12 pentalenolactone insensitive to Haloarcula vallismortis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.12 0.053
-
 D-glyceraldehyde 3-phosphate
-
 Haloarcula vallismortis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.12 K+ KCl, activity is enhanced by increasing concentrations of the salt Haloarcula vallismortis
1.2.1.12 LiCl activity is partly enhanced Haloarcula vallismortis
1.2.1.12 NaCl activity is enhanced by increasing concentrations of the salt Haloarcula vallismortis
1.2.1.12 NH4Cl activity is enhanced by increasing concentrations of the salt Haloarcula vallismortis
1.2.1.12 RbCl activity is enhanced by increasing concentrations of the salt. Optimum activity in 3 M RbCl Haloarcula vallismortis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.12 40000
-
 4 * 40000, SDS-PAGE Haloarcula vallismortis
1.2.1.12 190000
-
 gel filtration Haloarcula vallismortis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.12 D-glyceraldehyde 3-phosphate + phosphate + NAD+ Haloarcula vallismortis key enzyme of glycolysis 3-phospho-D-glyceroyl phosphate + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.12 Haloarcula vallismortis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.12
-
 Haloarcula vallismortis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.12 41.5
-
-
 Haloarcula vallismortis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.12 D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 Haloarcula vallismortis 3-phospho-D-glyceroyl phosphate + NADH
-
 ?
1.2.1.12 D-glyceraldehyde 3-phosphate + phosphate + NAD+ key enzyme of glycolysis Haloarcula vallismortis 3-phospho-D-glyceroyl phosphate + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.12 tetramer 4 * 40000, SDS-PAGE Haloarcula vallismortis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.12 additional information
-
 temperature of 50% inactivation increases by 15°C, when KCl concentration is increased from 1 M to 4 M or when 1 M KCl is replaced by 1 M (NH4)2SO4 Haloarcula vallismortis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.12 8.6
-
-
 Haloarcula vallismortis

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.12 NAD+
-
 Haloarcula vallismortis