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Literature summary extracted from

  • Kato, N.; Sahm, H.; Wagner, F.
    Steady-state kinetics of formaldehyde dehydrogenase and formate dehydrogenase from a methanol-utilizing yeast, Candida boidinii (1979), Biochim. Biophys. Acta, 566, 12-20.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.284 ADP competitive with respect to NAD+, noncompetitive with respect to S-hydroxymethylglutathione [Candida] boidinii
1.1.1.284 AMP competitive with respect to NAD+, noncompetitive with respect to S-hydroxymethylglutathione [Candida] boidinii
1.1.1.284 ATP competitive with respect to NAD+, noncompetitive with respect to S-hydroxymethylglutathione [Candida] boidinii
1.1.1.284 NADH
-
[Candida] boidinii
1.1.1.284 S-formylglutathione
-
[Candida] boidinii
1.1.1.284 S-hydroxymethylglutathione
-
[Candida] boidinii
1.17.1.9 ADP inhibition is greater at acidic pH than at neutral pH [Candida] boidinii
1.17.1.9 AMP
-
[Candida] boidinii
1.17.1.9 ATP inhibition is greater at acidic pH than at neutral pH [Candida] boidinii
1.17.1.9 CO2
-
[Candida] boidinii
1.17.1.9 NADH competitive versus NAD+ [Candida] boidinii
1.17.1.9 NADPH
-
[Candida] boidinii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.284 formaldehyde + glutathione + NAD+ [Candida] boidinii key enzyme of the dissimilatory pathway of the methanol metabolism S-formylglutathione + NADH + H+
-
?
1.17.1.9 formate + NAD+ [Candida] boidinii last enzyme of the dissimilatory pathway of the methanol metabolism CO2 + NADH + H+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.284 [Candida] boidinii
-
-
-
1.17.1.9 [Candida] boidinii
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.284 S-(hydroxymethyl)glutathione + NAD(P)+ = S-formylglutathione + NAD(P)H + H+ ordered bi-bi mechanism [Candida] boidinii
1.17.1.9 formate + NAD+ = CO2 + NADH + H+ ordered bi-bi mechanism, NAD+ is the first substrate and NADH is the last product [Candida] boidinii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.284 formaldehyde + glutathione + NAD+ the true substrate is a hemimercaptal, S-hydroxymethylglutathione, spontaneously formed from formaldehyde and glutathione [Candida] boidinii S-formylglutathione + NADH + H+
-
r
1.1.1.284 formaldehyde + glutathione + NAD+ key enzyme of the dissimilatory pathway of the methanol metabolism [Candida] boidinii S-formylglutathione + NADH + H+
-
?
1.1.1.284 S-formylglutathione + NADH
-
[Candida] boidinii formaldehyde + glutathione + NAD+
-
r
1.17.1.9 formate + NAD+
-
[Candida] boidinii CO2 + NADH + H+
-
?
1.17.1.9 formate + NAD+ last enzyme of the dissimilatory pathway of the methanol metabolism [Candida] boidinii CO2 + NADH + H+
-
?

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.284 NAD+
-
[Candida] boidinii
1.1.1.284 NADH
-
[Candida] boidinii
1.17.1.9 NAD+
-
[Candida] boidinii