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Literature summary extracted from

  • Kato, N.
    Formaldehyde dehydrogenase from methylotrophic yeasts (1990), Methods Enzymol., 188, 455-462.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.284 ADP
-
 [Candida] boidinii
1.1.1.284 Ag+
-
 [Candida] boidinii
1.1.1.284 AMP
-
 [Candida] boidinii
1.1.1.284 ATP
-
 [Candida] boidinii
1.1.1.284 Cd2+
-
 [Candida] boidinii
1.1.1.284 Cd2+
-
 Komagataella pastoris
1.1.1.284 Cu2+
-
 [Candida] boidinii
1.1.1.284 Cu2+
-
 Komagataella pastoris
1.1.1.284 Hg2+
-
 [Candida] boidinii
1.1.1.284 Hg2+
-
 Komagataella pastoris
1.1.1.284 NADH competitive inhibition, product inhibition [Candida] boidinii
1.1.1.284 NADH
-
 Komagataella pastoris
1.1.1.284 PCMB
-
 [Candida] boidinii
1.1.1.284 PCMB
-
 Komagataella pastoris
1.1.1.284 S-formylglutathione
-
 [Candida] boidinii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.284 0.025
-
 NAD+
-
 [Candida] boidinii
1.1.1.284 0.025
-
 NADH
-
 [Candida] boidinii
1.1.1.284 0.12
-
 S-formylglutathione
-
 [Candida] boidinii
1.1.1.284 0.29
-
 formaldehyde
-
 [Candida] boidinii
1.1.1.284 2.8
-
 methylglyoxal
-
 [Candida] boidinii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.284 43000
-
 2 * 43000, SDS-PAGE [Candida] boidinii
1.1.1.284 82000
-
 gel filtration [Candida] boidinii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.284 formaldehyde + glutathione + NAD+ Ogataea angusta
-
 S-formylglutathione + NADH + H+
-
 ?
1.1.1.284 formaldehyde + glutathione + NAD+ [Candida] boidinii key step of the methanol catabolism in yeast S-formylglutathione + NADH + H+
-
 ?
1.1.1.284 formaldehyde + glutathione + NAD+ [Candida] boidinii 2201 key step of the methanol catabolism in yeast S-formylglutathione + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.284 Komagataella pastoris
-
-
-
1.1.1.284 Ogataea angusta
-
-
-
1.1.1.284 Pichia sp.
-
 NRRL-Y-11328
-
1.1.1.284 Pichia sp. NRRL-Y-11328
-
 NRRL-Y-11328
-
1.1.1.284 [Candida] boidinii
-
-
-
1.1.1.284 [Candida] boidinii 2201
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.284
-
 [Candida] boidinii

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.284 S-(hydroxymethyl)glutathione + NAD(P)+ = S-formylglutathione + NAD(P)H + H+ bi bi mechanism [Candida] boidinii
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.284 38.3
-
-
 [Candida] boidinii

Storage Stability

EC Number Storage Stability Organism
1.1.1.284 -20°C, in presence of glycerol, stable for at least 6 months [Candida] boidinii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.284 formaldehyde + glutathione + NAD+
-
 Komagataella pastoris S-formylglutathione + NADH + H+
-
 r
1.1.1.284 formaldehyde + glutathione + NAD+
-
 Ogataea angusta S-formylglutathione + NADH + H+
-
 ?
1.1.1.284 formaldehyde + glutathione + NAD+
-
 Pichia sp. S-formylglutathione + NADH + H+
-
 ?
1.1.1.284 formaldehyde + glutathione + NAD+ the true substrate is a hemimercaptal, S-hydroxymethylglutathione, spontaneously formed from formaldehyde and glutathione [Candida] boidinii S-formylglutathione + NADH + H+
-
 r
1.1.1.284 formaldehyde + glutathione + NAD+ key step of the methanol catabolism in yeast [Candida] boidinii S-formylglutathione + NADH + H+
-
 ?
1.1.1.284 formaldehyde + glutathione + NAD+ the true substrate is a hemimercaptal, S-hydroxymethylglutathione, spontaneously formed from formaldehyde and glutathione [Candida] boidinii 2201 S-formylglutathione + NADH + H+
-
 r
1.1.1.284 formaldehyde + glutathione + NAD+ key step of the methanol catabolism in yeast [Candida] boidinii 2201 S-formylglutathione + NADH + H+
-
 ?
1.1.1.284 formaldehyde + glutathione + NAD+
-
 Pichia sp. NRRL-Y-11328 S-formylglutathione + NADH + H+
-
 ?
1.1.1.284 methylglyoxal + glutathione + NAD+
-
 Komagataella pastoris S-pyruvylglutathione + NADH
-
 ?
1.1.1.284 methylglyoxal + glutathione + NAD+ 89% of the activity with formaldehyde [Candida] boidinii S-pyruvylglutathione + NADH
-
 ?
1.1.1.284 methylglyoxal + glutathione + NAD+ 89% of the activity with formaldehyde [Candida] boidinii 2201 S-pyruvylglutathione + NADH
-
 ?
1.1.1.284 S-formylglutathione + NADH
-
 Komagataella pastoris formaldehyde + glutathione + NAD+
-
 r
1.1.1.284 S-formylglutathione + NADH
-
 [Candida] boidinii formaldehyde + glutathione + NAD+
-
 r
1.1.1.284 S-formylglutathione + NADH
-
 [Candida] boidinii 2201 formaldehyde + glutathione + NAD+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.284 dimer
-
 Komagataella pastoris
1.1.1.284 dimer 2 * 43000, SDS-PAGE [Candida] boidinii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.284 8
-
-
 Komagataella pastoris
1.1.1.284 8
-
-
 [Candida] boidinii
1.1.1.284 8
-
-
 Ogataea angusta
1.1.1.284 8.5
-
-
 Komagataella pastoris
1.1.1.284 8.5
-
-
 [Candida] boidinii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.284 6 10 20°C, 1 h, stable [Candida] boidinii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.284 NAD+
-
 Komagataella pastoris
1.1.1.284 NAD+
-
 [Candida] boidinii
1.1.1.284 NAD+
-
 Ogataea angusta
1.1.1.284 NAD+
-
 Pichia sp.
1.1.1.284 NADH
-
 Komagataella pastoris
1.1.1.284 NADH
-
 [Candida] boidinii
1.1.1.284 NADP+ no activity with NADP+ Komagataella pastoris
1.1.1.284 NADP+ no activity with NADP+ [Candida] boidinii