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Literature summary extracted from

  • Anderson, G.L.; Williams, J.; Hille, R.
    The purification and characterization of arsenite oxidase from Alcaligenes faecalis, a molybdenum-containing hydroxylase (1992), J. Biol. Chem., 267, 23674-23682.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.20.9.1 0.008
-
 azurin
-
 Alcaligenes faecalis
1.20.9.1 0.07
-
 arsenite
-
 Alcaligenes faecalis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.20.9.1 Fe contains 3 to 4 mol of Fe per 85 kD protein Alcaligenes faecalis
1.20.9.1 Mo contains 0.75 mol Mo per mol of protein Alcaligenes faecalis
1.20.9.1 Mo contains one Mo binding site Alcaligenes faecalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.20.9.1 85000
-
 gel filtration Alcaligenes faecalis

Organism

EC Number Organism UniProt Comment Textmining
1.20.9.1 Alcaligenes faecalis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.20.9.1
-
 Alcaligenes faecalis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.20.9.1 2.88
-
-
 Alcaligenes faecalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.20.9.1 arsenite + H2O + azurin(oxidized)
-
 Alcaligenes faecalis arsenate + azurin(reduced)
-
 ?

Subunits

EC Number Subunits Comment Organism
1.20.9.1 monomer SDS-PAGE Alcaligenes faecalis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.20.9.1 27
-
 azurin
-
 Alcaligenes faecalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.20.9.1 6
-
-
 Alcaligenes faecalis