Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Unemoto, T.; Hayashi, M.; Hayashi, M.
    Partial purification and properties of respiratory chain-linked l-glycerol 3-phosphate dehydrogenase from a marine bacterium, Vibrio alginolyticus (1981), J. Biochem., 90, 619-628.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.5.3 detergent required for maximal activity Vibrio alginolyticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.5.3 1,10-phenanthroline
-
 Vibrio alginolyticus
1.1.5.3 Ag+
-
 Vibrio alginolyticus
1.1.5.3 bathophenanthroline
-
 Vibrio alginolyticus
1.1.5.3 Hg2+
-
 Vibrio alginolyticus
1.1.5.3 PCMB
-
 Vibrio alginolyticus
1.1.5.3 Thenoyltrifluoroacetone
-
 Vibrio alginolyticus
1.1.5.3 Triton X100 in presence of phospholipids Vibrio alginolyticus
1.1.5.3 Zn2+
-
 Vibrio alginolyticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.5.3 0.13
-
 phenazine methosulfate
-
 Vibrio alginolyticus
1.1.5.3 0.55
-
 ferricyanide
-
 Vibrio alginolyticus
1.1.5.3 3.7
-
 sn-glycerol 3-phosphate with ferricyanide as electron acceptor Vibrio alginolyticus
1.1.5.3 4.02
-
 sn-glycerol 3-phosphate with phenazine methosulfate as electron acceptor Vibrio alginolyticus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.5.3 membrane
-
 Vibrio alginolyticus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.5.3 K+ 20 mM NaCl or KCl required for maximal activity Vibrio alginolyticus
1.1.5.3 Na+ 20 mM NaCl or KCl required for maximal activity Vibrio alginolyticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.5.3 300000
-
 gel filtration Vibrio alginolyticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.5.3 sn-glycerol 3-phosphate + acceptor Vibrio alginolyticus enzyme is linked to the respiratory chain glycerone phosphate + reduced acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.3 Vibrio alginolyticus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.5.3 partial Vibrio alginolyticus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.5.3 sn-glycerol 3-phosphate + a quinone = glycerone phosphate + a quinol ping-pong mechanism Vibrio alginolyticus
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.5.3 additional information
-
-
 Vibrio alginolyticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.3 sn-glycerol 3-phosphate + 2,6-dichlorophenolindophenol
-
 Vibrio alginolyticus glycerone phosphate + reduced 2,6-dichlorophenylindophenol
-
 ?
1.1.5.3 sn-glycerol 3-phosphate + acceptor enzyme is linked to the respiratory chain Vibrio alginolyticus glycerone phosphate + reduced acceptor
-
 ?
1.1.5.3 sn-glycerol 3-phosphate + coenzyme Q5
-
 Vibrio alginolyticus glycerone phosphate + reduced coenzyme Q5
-
 ?
1.1.5.3 sn-glycerol 3-phosphate + ferricyanide
-
 Vibrio alginolyticus glycerone phosphate + ferrocyanide
-
 ?
1.1.5.3 sn-glycerol 3-phosphate + phenazine methosulfate phenazine methosulfate-coupled reduction of thiazolyl blue tetrazolium Vibrio alginolyticus glycerone phosphate + reduced phenazine methosulfate
-
 ?
1.1.5.3 sn-glycerol 3-phosphate + phenazine methosulfate
-
 Vibrio alginolyticus glycerone phosphate + reduced phenazine methosulfat
-
 r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.5.3 8.5 9
-
 Vibrio alginolyticus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.5.3 FAD contains noncovalently bound FAD Vibrio alginolyticus