Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Fang, J.; Liu, W.; Gao, P.J.
    Cellobiose dehydrogenase from Schizophyllum commune: purification and study of some catalytic, inactivation, and cellulose-binding properties (1998), Arch. Biochem. Biophys., 353, 37-46.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.99.18 Ag+ 2 mM, 70% inhibition after 6 h Schizophyllum commune
1.1.99.18 Hg2+ 2 mM, 20% inhibition after 6 h Schizophyllum commune
1.1.99.18 SDS 5 mM, complete inhibition Schizophyllum commune
1.1.99.18 Zn2+ 2 mM, 60% inhibition after 6 h Schizophyllum commune

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.18 0.0028
-
cytochrome c
-
Schizophyllum commune
1.1.99.18 0.01
-
2,6-dichlorphenolindophenol
-
Schizophyllum commune
1.1.99.18 0.03
-
cellobiose
-
Schizophyllum commune
1.1.99.18 0.058
-
cellotriose
-
Schizophyllum commune
1.1.99.18 0.06
-
cellotetraose
-
Schizophyllum commune
1.1.99.18 0.071
-
ferricyanide
-
Schizophyllum commune
1.1.99.18 0.085
-
cellopentaose
-
Schizophyllum commune
1.1.99.18 0.108
-
lactose
-
Schizophyllum commune

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.99.18 extracellular
-
Schizophyllum commune
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.99.18 102000
-
x * 102000, SDS-PAGE Schizophyllum commune

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.18 Schizophyllum commune
-
white-rot fungus
-
1.1.99.18 Schizophyllum commune AS 5.391
-
white-rot fungus
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.1.99.18 very stable against H2O2 inactivation, 25 mM H2O2, pH 5.0, no loss in activity after 24 h, at 50 mM H2O2 27% enzyme activity are lost within 12 h, at 1 mM H2O2 with addition of 2 mM cellobiose and 0.05 mM FeCl3 all enzyme activity is lost within 4 h Schizophyllum commune

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.99.18 glycoprotein 14% carbohydrate content Schizophyllum commune

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.18 ammonium sulfate, DEAE-Sephadex A50, Sephadex G-100 Schizophyllum commune

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.99.18 culture medium
-
Schizophyllum commune
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.99.18 11.4
-
-
Schizophyllum commune

Storage Stability

EC Number Storage Stability Organism
1.1.99.18 4°C, several months Schizophyllum commune

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.18 cellobiose + 2,6-dichlorophenolindophenol
-
Schizophyllum commune cellobiono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
r
1.1.99.18 cellobiose + acceptor electron acceptors: cytochrome c, 2,6-dichlorphenolindophenol, ferricyanide. O2 is reduced to H2O2 Schizophyllum commune cellobiono-1,5-lactone + reduced acceptor
-
?
1.1.99.18 cellobiose + acceptor electron acceptors: cytochrome c, 2,6-dichlorphenolindophenol, ferricyanide. O2 is reduced to H2O2 Schizophyllum commune AS 5.391 cellobiono-1,5-lactone + reduced acceptor
-
?
1.1.99.18 cellobiose + cytochrome c
-
Schizophyllum commune cellobiono-1,5-lactone + reduced cytochrome c
-
r
1.1.99.18 cellobiose + ferricyanide
-
Schizophyllum commune cellobiono-1,5-lactone + ferrocyanide
-
r
1.1.99.18 cellopentaose + acceptor
-
Schizophyllum commune cellopentaono-1,5-lactone + reduced acceptor
-
?
1.1.99.18 cellopentaose + acceptor
-
Schizophyllum commune AS 5.391 cellopentaono-1,5-lactone + reduced acceptor
-
?
1.1.99.18 cellotetraose + acceptor
-
Schizophyllum commune cellotetrono-1,5-lactone + reduced acceptor
-
?
1.1.99.18 cellotetraose + acceptor
-
Schizophyllum commune AS 5.391 cellotetrono-1,5-lactone + reduced acceptor
-
?
1.1.99.18 cellotriose + acceptor
-
Schizophyllum commune cellotriono-1,5-lactone + reduced acceptor
-
?
1.1.99.18 cellotriose + acceptor
-
Schizophyllum commune AS 5.391 cellotriono-1,5-lactone + reduced acceptor
-
?
1.1.99.18 lactose + acceptor
-
Schizophyllum commune 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
-
?
1.1.99.18 lactose + acceptor
-
Schizophyllum commune AS 5.391 4-O-(beta-D-galactopyranosyl)-D-glucono-1,5-lactone + reduced acceptor
-
?

Subunits

EC Number Subunits Comment Organism
1.1.99.18 ? x * 102000, SDS-PAGE Schizophyllum commune

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.99.18 35
-
loss of 40% activity after 8 h at 45°C, enzyme is almost completely inactivated after 4 h at 50° Schizophyllum commune

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.99.18 6
-
lactose
-
Schizophyllum commune
1.1.99.18 10
-
cellopentaose
-
Schizophyllum commune
1.1.99.18 12
-
cellotriose
-
Schizophyllum commune
1.1.99.18 12
-
cellotetraose
-
Schizophyllum commune
1.1.99.18 13
-
cellobiose
-
Schizophyllum commune
1.1.99.18 13.5
-
ferricyanide
-
Schizophyllum commune
1.1.99.18 25
-
cytochrome c
-
Schizophyllum commune
1.1.99.18 63
-
2,6-dichlorphenolindophenol
-
Schizophyllum commune

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.99.18 4.5
-
-
Schizophyllum commune

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.99.18 4 11 at 30°C stable for 24 h, at pH 3.5 almost 40% activity are lost within 12 h Schizophyllum commune

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.99.18 FAD
-
Schizophyllum commune
1.1.99.18 heme b
-
Schizophyllum commune