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Literature summary extracted from
- Enzymatic studies on the oxidation of sugar and sugar alcohol. 8. Particle-bound L-sorbose dehydrogenase from Gluconobacter suboxydans (1969), J. Biochem., 66, 521-527.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.99.12 | azide | 1 mM, 30-40% increase in activity, 2,6-dichlorophenol as electron acceptor | Gluconobacter oxydans |  |
| 1.1.99.12 | cyanide | 1 mM, 30-40% increase in activity, 2,6-dichlorophenol as electron acceptor | Gluconobacter oxydans | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.99.12 | Ag+ | 1 mM, complete inhibition | Gluconobacter oxydans | |
| 1.1.99.12 | Cu2+ | 1 mM, complete inhibition | Gluconobacter oxydans | |
| 1.1.99.12 | Hg2+ | 1 mM, complete inhibition | Gluconobacter oxydans | |
| 1.1.99.12 | p-chloromercuribenzoate | 1 mM, 40% inhibition | Gluconobacter oxydans | |
| 1.1.99.12 | Phenylmercuric nitrate | 1 mM, 40% inhibition | Gluconobacter oxydans | |
| 1.1.99.12 | Semicarbazide | 1 mM, 40% inhibition | Gluconobacter oxydans | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.99.12 | 4.3 | - |
L-sorbose | - |
Gluconobacter oxydans | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.1.99.12 | particle-bound | - |
Gluconobacter oxydans | - |
- |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.99.12 | L-sorbose + 2,6-dichlorophenolindophenol | Gluconobacter oxydans | no activity with D-fructose, sucrose and dulcitol | 5-keto-D-fructose + reduced 2,6-dichlorophenolindophenol | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.99.12 | Gluconobacter oxydans | - |
- |
- |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.12 | 0.0099 | - |
- |
Gluconobacter oxydans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.99.12 | D-galactose + 2,6-dichlorophenolindophenol | 180% of activity with L-sorbose | Gluconobacter oxydans | ? | - |
? | |
| 1.1.99.12 | D-gluconate + 2,6-dichlorophenolindophenol | 135% of activity with L-sorbose | Gluconobacter oxydans | ? | - |
? | |
| 1.1.99.12 | D-glucose + 2,6-dichlorophenolindophenol | 382% of activity with L-sorbose | Gluconobacter oxydans | 2-dehydro-D-glucose + reduced 2,6-dichlorophenolindophenol | - |
? | |
| 1.1.99.12 | D-mannitol + 2,6-dichlorophenolindophenol | 328% of activity with L-sorbose | Gluconobacter oxydans | ? | - |
? | |
| 1.1.99.12 | D-mannose + 2,6-dichlorophenolindophenol | 190% of activity with L-sorbose | Gluconobacter oxydans | ? | - |
? | |
| 1.1.99.12 | D-sorbitol + 2,6-dichlorophenolindophenol | 728% of activity with L-sorbose | Gluconobacter oxydans | ? | - |
? | |
| 1.1.99.12 | D-xylose + 2,6-dichlorophenolindophenol | 355% of activity with L-sorbose | Gluconobacter oxydans | ? | - |
? | |
| 1.1.99.12 | glycerol + 2,6-dichlorophenolindophenol | 327% of activity with L-sorbose | Gluconobacter oxydans | ? | - |
? | |
| 1.1.99.12 | iso-erythritol + 2,6-dichlorophenolindophenol | 236% of activity with L-sorbose | Gluconobacter oxydans | ? | - |
? | |
| 1.1.99.12 | L-arabinose + 2,6-dichlorophenolindophenol | 318% of activity with L-sorbose | Gluconobacter oxydans | ? | - |
? | |
| 1.1.99.12 | L-sorbose + 2,6-dichlorophenolindophenol | no activity with D-fructose, sucrose and dulcitol | Gluconobacter oxydans | 5-keto-D-fructose + reduced 2,6-dichlorophenolindophenol | - |
? | |
| 1.1.99.12 | maltose + 2,6-dichlorophenolindophenol | 282% of activity with L-sorbose | Gluconobacter oxydans | 4-O-alpha-D-ribo-hexopyranosyl-3-ulose-beta-D-glucopyranose + reduced 2,6-dichlorophenolindophenol | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.12 | 20 | - |
assay at | Gluconobacter oxydans |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.12 | 40 | - |
5 min, complete inactivation | Gluconobacter oxydans |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.12 | 6.4 | - |
- |
Gluconobacter oxydans |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.12 | 5 | 8.7 | pH 5.0: about 40% of activity maximum, pH 8.7: about 30% of activity maximum | Gluconobacter oxydans |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.99.12 | 4 | - |
30 min, 20°C, complete inactivation | Gluconobacter oxydans |
| 1.1.99.12 | 6 | 6.5 | 30 min, 20°C, highest stability | Gluconobacter oxydans |
| 1.1.99.12 | 7.5 | - |
30 min, 20°C, complete inactivation | Gluconobacter oxydans |
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