EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.3.6 | expression in Escherichia coli | Streptomyces sp. |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.3.6 | R135H | random mutagenesis | Streptomyces sp. |
1.1.3.6 | S103T | random mutagenesis | Streptomyces sp. |
1.1.3.6 | V121A | random mutagenesis | Streptomyces sp. |
1.1.3.6 | V145E | random mutagenesis | Streptomyces sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.3.6 | 0.011 | - |
cholesterol | V145E mutant | Streptomyces sp. | |
1.1.3.6 | 0.013 | - |
cholesterol | wild-type | Streptomyces sp. | |
1.1.3.6 | 0.013 | - |
cholesterol | S103T mutant | Streptomyces sp. | |
1.1.3.6 | 0.03 | - |
cholesterol | R135H mutant | Streptomyces sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.3.6 | cholesterol + O2 | Streptomyces sp. | - |
cholest-5-en-3-one + H2O2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.3.6 | Streptomyces sp. | - |
- |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.1.3.6 | cholesterol + O2 = cholest-5-en-3-one + H2O2 | bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid | Streptomyces sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.3.6 | 26.7 | - |
S103T/V145A/V121A/R135H quadruple mutant | Streptomyces sp. |
1.1.3.6 | 39.9 | - |
S103T/V145A double mutant | Streptomyces sp. |
1.1.3.6 | 47.8 | - |
S103T/V145A/V121A triple mutant | Streptomyces sp. |
1.1.3.6 | 63.5 | - |
V145E mutant, 37°C, pH 7.0 | Streptomyces sp. |
1.1.3.6 | 67.7 | - |
wild type | Streptomyces sp. |
1.1.3.6 | 68.9 | - |
R135H mutant, 37°C, pH 7.0 | Streptomyces sp. |
1.1.3.6 | 72 | - |
V121A mutant, 37°C, pH 7.0 | Streptomyces sp. |
1.1.3.6 | 86.6 | - |
S103T mutant, 37°C, pH 7.0 | Streptomyces sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.3.6 | cholesterol + O2 | - |
Streptomyces sp. | cholest-4-en-3-one + H2O2 | - |
? | |
1.1.3.6 | cholesterol + O2 | - |
Streptomyces sp. | cholest-5-en-3-one + H2O2 | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.3.6 | 60 | - |
wild-type and S103T, V121A, R135H and V145E mutants | Streptomyces sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.3.6 | 60 | - |
half-lives of S103T, V121A, R135H, V145E mutants and wild-type: 11.3, 12.2, 11.0, 24.1 and 7.8 min respectively, half-lives of S103T/V145A double mutants and S103T/V145A/V121A triple mutants: 32.4 and 52.2 min respectively | Streptomyces sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.3.6 | 6 | - |
wild-type, S103T, V121A and R135H mutants | Streptomyces sp. |
1.1.3.6 | 7.5 | - |
V145E mutant | Streptomyces sp. |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.3.6 | 6 | 8 | wild-type, S103T, V121A, R135H and V145E mutants | Streptomyces sp. |