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Literature summary extracted from

  • Brooks, C.J.W.; Smith, A.G.
    Cholesterol oxidase. Further studies of substrate specificity in relation to the analytical characterisation of steroids (1975), J. Chromatogr., 112, 499-511.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.6 0.0012
-
 26-Hydroxycholesterol
-
 Nocardia erythropolis
1.1.3.6 0.0015
-
 25-hydroxycholesterol
-
 Nocardia erythropolis
1.1.3.6 0.0027
-
 24-oxocholesterol
-
 Nocardia erythropolis
1.1.3.6 0.0029
-
 cholesterol
-
 Nocardia erythropolis
1.1.3.6 0.0042
-
 20-hydroxycholesterol
-
 Nocardia erythropolis
1.1.3.6 0.0043
-
 24-hydroxycholesterol
-
 Nocardia erythropolis
1.1.3.6 0.0051
-
 22-hydroxycholesterol
-
 Nocardia erythropolis
1.1.3.6 0.0071
-
 20,22-dihydroxycholesterol
-
 Nocardia erythropolis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.6 cholesterol + O2 Nocardia erythropolis
-
 cholest-5-en-3-one + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.6 Nocardia erythropolis
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.3.6 cholesterol + O2 = cholest-5-en-3-one + H2O2 bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid Nocardia erythropolis
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.6 20,22-dihydroxycholesterol + O2
-
 Nocardia erythropolis 20,22-dihydroxycholest-5-en-3-one + H2O2
-
 ?
1.1.3.6 20-hydroxycholesterol + O2
-
 Nocardia erythropolis 20-hydroxycholest-5-en-3-one + H2O2
-
 ?
1.1.3.6 22-hydroxycholesterol + O2
-
 Nocardia erythropolis 22-hydroxycholest-5-en-3-one + H2O2
-
 ?
1.1.3.6 24-hydroxycholesterol + O2
-
 Nocardia erythropolis 24-hydroxycholest-5-en-3-one + H2O2
-
 ?
1.1.3.6 24-oxocholesterol + O2
-
 Nocardia erythropolis 24-oxocholest-5-en-3-one + H2O2
-
 ?
1.1.3.6 25-hydroxycholesterol + O2
-
 Nocardia erythropolis 25-hydroxycholest-5-en-3-one + H2O2
-
 ?
1.1.3.6 26-hydroxycholesterol + O2
-
 Nocardia erythropolis 26-hydroxycholest-5-en-3-one + H2O2
-
 ?
1.1.3.6 cholesterol + O2 overview: substrate specificity Nocardia erythropolis cholest-4-en-3-one + H2O2
-
 ?
1.1.3.6 cholesterol + O2 relatively unreactive 17keto-DELTA5-3beta-hydroxysteroids are converted to satisfactory substrates by formation of isopentyloximes or benzyloximes Nocardia erythropolis cholest-4-en-3-one + H2O2
-
 ?
1.1.3.6 cholesterol + O2 size and shape of steroid 17beta-side-chain and oxygenation of nucleus Nocardia erythropolis cholest-4-en-3-one + H2O2
-
 ?
1.1.3.6 cholesterol + O2
-
 Nocardia erythropolis cholest-5-en-3-one + H2O2
-
 ?