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Literature summary extracted from

  • Fukuyama, M.; Miyake, Y.
    Purification and some properties of cholesterol oxidase from Schizophyllum commune with covalently bound flavin (1979), J. Biochem., 85, 1183-1193.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.3.6 Triton X-100 inactivates at a concentration of 0.02% Schizophyllum commune

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.6 sodium deoxycholate
-
 Schizophyllum commune
1.1.3.6 Triton X-100 inactivates at a concentration of 0.02% Schizophyllum commune

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.6 0.33
-
 cholesterol
-
 Schizophyllum commune

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.6 53000
-
 SDS-PAGE, sedimentation equilibrium, amino acid analysis Schizophyllum commune

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.6 cholesterol + O2 Schizophyllum commune
-
 cholest-5-en-3-one + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.6 Schizophyllum commune
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.6
-
 Schizophyllum commune

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.3.6 cholesterol + O2 = cholest-5-en-3-one + H2O2 bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid Schizophyllum commune
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.6 cholesterol + O2
-
 Schizophyllum commune cholest-4-en-3-one + H2O2
-
 ?
1.1.3.6 cholesterol + O2
-
 Schizophyllum commune cholest-5-en-3-one + H2O2
-
 ?
1.1.3.6 dehydroepiandrosterone + O2
-
 Schizophyllum commune androst-5-en-3,17-dione + H2O2
-
 ?
1.1.3.6 lanosterol + O2 slow rate Schizophyllum commune lanosta-8,24-dien-3-one + H2O2
-
 ?
1.1.3.6 pregnenolone + O2
-
 Schizophyllum commune 5-pregnen-3,20-dione + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.6 monomer 1 * 53000, SDS-PAGE Schizophyllum commune

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.6 4 5
-
 Schizophyllum commune

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.6 FAD firmly bound Schizophyllum commune
1.1.3.6 FAD flavoprotein Schizophyllum commune