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Literature summary extracted from

  • Yoshimoto, T.; Ritani, A.; Ohwada, K.; Takahashi, K.; Kodera, Y.; Matsushima, A.; Saito, Y.; Inada, Y.
    Polyethylene glycol derivative-modified cholesterol oxidase soluble and active in benzene (1987), Biochem. Biophys. Res. Commun., 148, 876-882.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.6 cholesterol + O2 Nocardia sp.
-
 cholest-5-en-3-one + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.6 Nocardia sp.
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.3.6 cholesterol + O2 = cholest-5-en-3-one + H2O2 bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid Nocardia sp.
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.6 0.6
-
 polyethylene glycol derivative-modified enzyme Nocardia sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.6 beta-sitosterol + O2
-
 Nocardia sp. stigmast-5-en-3-one + H2O2
-
 ?
1.1.3.6 cholesterol + O2
-
 Nocardia sp. cholest-4-en-3-one + H2O2
-
 ?
1.1.3.6 cholesterol + O2
-
 Nocardia sp. cholest-5-en-3-one + H2O2
-
 ?
1.1.3.6 fucosterol + O2
-
 Nocardia sp. stigmasta-5,24(28)-dien-3-one + H2O2
-
 ?