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Literature summary extracted from

  • Uwajima, T.; Yagi, H.; Terado, O.
    Properties of crystalline 3beta-hydroxysteroid oxidase of Brevibacterium sterolicum (1974), Agric. Biol. Chem., 38, 1149-1156.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.6 AgNO3 1 mM, 100% inhibition, 0.1 mM, 62% inhibition Brevibacterium sterolicum
1.1.3.6 HgCl2 1 mM, 68% inhibition, 0.1 mM, 38% inhibition Brevibacterium sterolicum
1.1.3.6 p-chloromercuribenzoate 1 mM, 20% inhibition, 0.1 mM, 14% inhibition Brevibacterium sterolicum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.3.6 additional information contains no metal ions Brevibacterium sterolicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.6 32500
-
 gel filtration, sedimentation equilibrium Brevibacterium sterolicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.6 cholesterol + O2 Brevibacterium sterolicum
-
 cholest-5-en-3-one + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.6 Brevibacterium sterolicum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.6
-
 Brevibacterium sterolicum

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.3.6 cholesterol + O2 = cholest-5-en-3-one + H2O2 bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid Brevibacterium sterolicum
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.3.6 cell culture
-
 Brevibacterium sterolicum
-

Storage Stability

EC Number Storage Stability Organism
1.1.3.6 0-5°C, crystallized enzyme in lyophilized state, in the dark, stable for at least 10 months Brevibacterium sterolicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.6 5alpha-cholestan-3beta-ol + O2 13.2% of activity with cholesterol Brevibacterium sterolicum 5alpha-cholestan-3-one + H2O2
-
 ?
1.1.3.6 beta-sitosterol + O2 19.7% of activity with cholesterol Brevibacterium sterolicum stigmast-5-en-3-one + H2O2
-
 ?
1.1.3.6 cholesterol + O2
-
 Brevibacterium sterolicum cholest-4-en-3-one + H2O2
-
 ?
1.1.3.6 cholesterol + O2
-
 Brevibacterium sterolicum cholest-5-en-3-one + H2O2
-
 ?
1.1.3.6 dehydroepiandrosterone + O2 41.4% of the activity with cholesterol Brevibacterium sterolicum androst-5-en-3,17-dione + H2O2
-
 ?
1.1.3.6 pregnenolone + O2 22.4% of activity with chelesterol Brevibacterium sterolicum 5-pregnen-3,20-dione + H2O2
-
 ?
1.1.3.6 stigmasterol + O2 10% of activity with cholesterol Brevibacterium sterolicum stigmasta-5,22-dien-3-one + H2O2
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.6 50
-
 pH 7.0, 30 min, stable Brevibacterium sterolicum
1.1.3.6 60
-
 pH 7.0, 30 min, 19% loss of activity Brevibacterium sterolicum
1.1.3.6 70
-
 pH 7, 30 min, about 94% loss of activity Brevibacterium sterolicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.6 7.5
-
-
 Brevibacterium sterolicum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.3.6 5 9.5 about 50% of maximal activity at pH 5 and 9.5 Brevibacterium sterolicum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.3.6 4 10 37°C, 30 min, stable Brevibacterium sterolicum
1.1.3.6 5 10 37°C, 30 min, stable Brevibacterium sterolicum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.6 FAD flavoprotein Brevibacterium sterolicum
1.1.3.6 FAD 1 mol of FAD per mol of protein Brevibacterium sterolicum