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Literature summary extracted from

  • Cheetham, P.S.J.; Dunnill, P.; Lilly, M.D.
    The characterization and interconversion of three forms of cholesterol oxidase extracted from Nocardia rhodochrous (1982), Biochem. J., 201, 515-521.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.3.6 Triton X-100 stimulates at concentration up to 0.2% Nocardia rhodochrous

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.6 cholest-4-en-3-one
-
Nocardia rhodochrous
1.1.3.6 HgCl2 10 mM, complete inhibition Nocardia rhodochrous

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.6 cholesterol + O2 Nocardia rhodochrous first step in cholesterol degradation cholest-5-en-3-one + H2O2
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.6 Nocardia rhodochrous
-
3 forms
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.3.6 additional information 3 enzyme forms differ chiefly in the presence or absence of hydrophobic anchor region connected by a trypsin-sensitive region, no phospholipids are extracted with the enzyme Nocardia rhodochrous

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.3.6 cholesterol + O2 = cholest-5-en-3-one + H2O2 bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid Nocardia rhodochrous

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.6 0.045 0.069 substrate cholesterol Nocardia rhodochrous

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.6 cholesterol + O2
-
Nocardia rhodochrous cholest-4-en-3-one + H2O2
-
?
1.1.3.6 cholesterol + O2 first step in cholesterol degradation Nocardia rhodochrous cholest-5-en-3-one + H2O2
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.6 30
-
-
Nocardia rhodochrous

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.3.6 13 44 about 50% of maximal activity at 13°C and 44°C Nocardia rhodochrous

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.6 50
-
60 h, about 25% loss of activity Nocardia rhodochrous
1.1.3.6 80
-
5 min, about 70% loss of activity Nocardia rhodochrous

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.6 7
-
-
Nocardia rhodochrous

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.3.6 4.5 8 about 50% of maximal activity at pH 4.5, about 90% of maximal activity at pH 8 Nocardia rhodochrous