Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Zannoni, V.G.; Weber, W.W.
    Isolation and properties of aromatic alpha-keto acid reductase (1966), J. Biol. Chem., 241, 1340-1344.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.96 2,5-dihydroxybenzoic acid 1 mM, 44% inhibition Bos taurus
1.1.1.96 2,5-dihydroxyphenyllactic acid 1 mM, 20% inhibition Bos taurus
1.1.1.96 3,5-Diiodo-4-hydroxyphenyllactic acid 1 mM, 18% inhibition Bos taurus
1.1.1.96 3,5-diiodotyrosine 1 mM, 15% inhibition Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.96 0.043
-
 3,5-diiodo-4-hydroxyphenylpyruvate
-
 Bos taurus
1.1.1.96 0.052
-
 NADH
-
 Bos taurus
1.1.1.96 1.9
-
 4-hydroxyphenylpyruvate
-
 Bos taurus
1.1.1.96 4.8
-
 phenylpyruvate
-
 Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.96 Bos taurus
-
-
-
1.1.1.96 Canis lupus familiaris
-
-
-
1.1.1.96 Cavia porcellus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.96 ammonium sulfate, calcium phosphate gel Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.96 adrenal gland
-
 Cavia porcellus
-
1.1.1.96 blood
-
 Cavia porcellus
-
1.1.1.96 brain
-
 Cavia porcellus
-
1.1.1.96 brain
-
 Canis lupus familiaris
-
1.1.1.96 heart
-
 Cavia porcellus
-
1.1.1.96 heart
-
 Bos taurus
-
1.1.1.96 heart
-
 Canis lupus familiaris
-
1.1.1.96 kidney
-
 Cavia porcellus
-
1.1.1.96 kidney
-
 Canis lupus familiaris
-
1.1.1.96 liver
-
 Cavia porcellus
-
1.1.1.96 liver
-
 Canis lupus familiaris
-
1.1.1.96 muscle
-
 Cavia porcellus
-
1.1.1.96 thyroid gland
-
 Cavia porcellus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.96 41.75
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
1.1.1.96 5°C, 160 mM phosphate, pH 6.5, 1 month, 20% activity lost Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.96 3,5-dinitro-4-hydroxyphenylpyruvate + NADH
-
 Bos taurus 3,5-dinitro-4-hydroxyphenyllactate + NAD+
-
 r
1.1.1.96 3-(3,5-dibromo-4-hydroxyphenyl)pyruvate + NADH
-
 Bos taurus 3-(3,5-dibromo-4-hydroxyphenyl)lactate + NAD+
-
 r
1.1.1.96 3-(3,5-diiodo-4-hydroxyphenyl)pyruvate + NADH
-
 Canis lupus familiaris 3-(3,5-diiodo-4-hydroxyphenyl)lactate + NAD+
-
 r
1.1.1.96 3-(3,5-diiodo-4-hydroxyphenyl)pyruvate + NADH formation of phenyllactic acids is strongly favoured Bos taurus 3-(3,5-diiodo-4-hydroxyphenyl)lactate + NAD+
-
 r
1.1.1.96 3-bromo-4-hydroxyphenylpyruvate + NADH
-
 Bos taurus 3-bromo-4-hydroxyphenyllactate + NAD+
-
 r
1.1.1.96 3-fluorophenylpyruvate + NADH
-
 Bos taurus 3-fluorophenyllactate + NAD+
-
 r
1.1.1.96 4-hydroxyphenylpyruvate + NADH + H+
-
 Bos taurus 4-hydroxyphenyllactate + NAD+
-
 r
1.1.1.96 indolepyruvate + NADH
-
 Bos taurus indolelactate + NAD+
-
 r
1.1.1.96 indolepyruvate + NADH
-
 Canis lupus familiaris indolelactate + NAD+
-
 r
1.1.1.96 phenylpyruvate + NADH
-
 Bos taurus phenyllactate + NAD+
-
 r
1.1.1.96 phenylpyruvate + NADH
-
 Canis lupus familiaris phenyllactate + NAD+
-
 r

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.96 62
-
 not completely inactivated after 2 min, completely inactivated above 72°C Bos taurus
1.1.1.96 72
-
 complete inactivation in 2 min Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.96 6 7
-
 Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.96 NADH
-
 Bos taurus
1.1.1.96 NADH
-
 Canis lupus familiaris
1.1.1.96 NADPH 15% activity compared to NADH Bos taurus