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Literature summary extracted from

  • Kleczkowski, L.A.; Randall, D.D.
    Purification and characterization of a novel NADPH(NADH)-dependent hydroxypyruvate reductase from spinach leaves. Comparison of immunological properties of leaf hydroxypyruvate reductases (1988), Biochem. J., 250, 145-152.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.81 additional information not inhibited by 2 mM acetohydroxamate Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.81 0.8
-
 Hydroxypyruvate at pH 6.0 Spinacia oleracea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.81 38000
-
 2 * 38000, SDS-PAGE Spinacia oleracea
1.1.1.81 70000
-
 gel filtration Spinacia oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.81 hydroxypyruvate + NAD(P)H Spinacia oleracea
-
 D-glycerate + NAD(P)+ + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.81 Spinacia oleracea
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.81 leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.81 257
-
-
 Spinacia oleracea

Storage Stability

EC Number Storage Stability Organism
1.1.1.81 -20°C, 20 mM MOPS, pH 7.1, 14 mM 2-mercaptoethanol, 50% glycerol Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.81 glyoxylate + NAD(P)H activity with hydroxypyruvate and NADPH is 2fold higher than with other pair of reactants Spinacia oleracea glycolate + NAD(P)+
-
 ir
1.1.1.81 hydroxypyruvate + NAD(P)H
-
 Spinacia oleracea D-glycerate + NAD(P)+ + H+
-
 ?
1.1.1.81 hydroxypyruvate + NAD(P)H
-
 Spinacia oleracea D-glycerate + NAD(P)+ + H+
-
 ir

Subunits

EC Number Subunits Comment Organism
1.1.1.81 dimer 2 * 38000, SDS-PAGE Spinacia oleracea

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.81 5.5 6 strong decrease above Spinacia oleracea