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Literature summary extracted from

  • Koekemoer, T.C.; Litthauer, D.; Oelofsen, W.
    Isolation and characterization of adipose tissue glycerol-3-phasphate dehydrogenase (1995), Int. J. Biochem. Cell Biol., 27, 625-632.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.8 glycerol competitive activator with respect to dihydroxyacetone phosphate above 30 mM Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.8 iodoacetamide effective inhibitor Homo sapiens
1.1.1.8 iodoacetate 50 mM, incubated for 60 min Homo sapiens
1.1.1.8 NADPH
-
 Homo sapiens
1.1.1.8 p-chloromercuribenzoate effective inhibitor Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.8 35000
-
 2 * 35000, SDS-PAGE Homo sapiens
1.1.1.8 74000
-
 electrophoresis in a non-denaturing system Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.8 sn-glycerol 3-phosphate + NAD+ Homo sapiens
-
 dihydroxyacetone phosphate + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.8 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.8 affinity chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.8 adipose tissue
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.8 250
-
-
 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
1.1.1.8 -20°C, 50% glycerol, retains activity for several months Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.8 sn-glycerol 3-phosphate + NAD+
-
 Homo sapiens dihydroxyacetone phosphate + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.8 dimer 2 * 35000, SDS-PAGE Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.8 7.5
-
 dihydroxyacetone phosphate reduction Homo sapiens
1.1.1.8 9
-
 glycerol-3-phosphate oxidation Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.8 additional information no activity with NADPH Homo sapiens
1.1.1.8 NAD+
-
 Homo sapiens
1.1.1.8 NADH
-
 Homo sapiens