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Literature summary extracted from

  • Narise, S.
    Purification and biochemical properties of allelic forms of cytoplasmic glycerol-3-phosphate dehydrogenase from Drosophila virilis (1980), Biochim. Biophys. Acta, 615, 289-298.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.8 dihydroxyacetone phosphate 0.5 mM, GPDHs more susceptible to inhibition Drosophila virilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.8 additional information
-
 additional information Km values of two further allelic isozymes, alpha-GPDHf and alpha-GPDHm Drosophila virilis
1.1.1.8 0.00526
-
 NADH alpha-GPDHs Drosophila virilis
1.1.1.8 0.1003
-
 NAD+ alpha-GPDHs, standard deviation 0.0041 mM Drosophila virilis
1.1.1.8 0.2144
-
 dihydroxyacetone phosphate alpha-GPDHs, standard deviation 0.0215 mM Drosophila virilis
1.1.1.8 1.89
-
 glycerol 3-phosphate alpha-GPDHs, standard deviation 0.25 mM Drosophila virilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.8 65000
-
 gel filtration Drosophila virilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.8 sn-glycerol 3-phosphate + NAD+ Drosophila virilis
-
 dihydroxyacetone phosphate + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.8 Drosophila virilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.8 3 allelic forms distinguishable by electrophoresis Drosophila virilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.8 71.8
-
-
 Drosophila virilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.8 sn-glycerol 3-phosphate + NAD+
-
 Drosophila virilis dihydroxyacetone phosphate + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.8 dimer 2 * 35000-37000, SDS-PAGE Drosophila virilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.8 30
-
 isozyme GPDHf, activity falls off at temperature above 30°C Drosophila virilis
1.1.1.8 40
-
 isozyme GPDHm and GPDHs Drosophila virilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.8 35
-
 most stable at pH 8, activity reduced, GPDHf to a greater extent Drosophila virilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.8 6 6.5 maximal activities of the three allelic forms are obtained within a pH range of 6.0-6.5 for dihydroxyacetone phosphate reduction Drosophila virilis
1.1.1.8 10 10.5 maximal activities of the three allelic forms are obtained within a pH range of 10.0-10.5 for glycerol 3-phosphate oxidation Drosophila virilis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.8 7
-
 rate of inactivation increases below pH 7 Drosophila virilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.8 NAD+
-
 Drosophila virilis
1.1.1.8 NADH
-
 Drosophila virilis