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Literature summary extracted from

  • Kleczkowski, L.A.; Randall, D.D.; Blevins, D.G.
    Purification and characterization of a novel NADPH(NADH)-dependent glyoxylate reductase from spinach leaves. Comparison of immunological properties of leaf glyoxylate reductase and hydroxypyruvate reductase (1986), Biochem. J., 239, 653-659.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.79 0.003
-
 NADPH
-
 Spinacia oleracea
1.1.1.79 0.085
-
 glyoxylate cofactor NADPH Spinacia oleracea
1.1.1.79 0.15
-
 NADH
-
 Spinacia oleracea
1.1.1.79 1.1
-
 glyoxylate cofactor NADH Spinacia oleracea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.79 33000
-
 4 * 33000, SDS-PAGE Spinacia oleracea
1.1.1.79 125000
-
 gel filtration Spinacia oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.79 glyoxylate + NAD(P)H Triticum aestivum
-
 glycolate + NAD(P)+
-
 ?
1.1.1.79 glyoxylate + NAD(P)H Spinacia oleracea
-
 glycolate + NAD(P)+
-
 ?
1.1.1.79 glyoxylate + NAD(P)H Glycine max
-
 glycolate + NAD(P)+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.79 Glycine max
-
-
-
1.1.1.79 Spinacia oleracea
-
-
-
1.1.1.79 Triticum aestivum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.79 ammonium sulfate, affinity chromatography, Sephadex G-75SF, AgdApP-4 Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.79 leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.79 0.45 0.7 glyoxylate reduction in crude extracts Triticum aestivum
1.1.1.79 0.45 0.7 glyoxylate reduction in crude extracts Glycine max
1.1.1.79 210.6
-
-
 Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.79 glyoxylate + NAD(P)H
-
 Triticum aestivum glycolate + NAD(P)+
-
 ?
1.1.1.79 glyoxylate + NAD(P)H
-
 Spinacia oleracea glycolate + NAD(P)+
-
 ?
1.1.1.79 glyoxylate + NAD(P)H
-
 Glycine max glycolate + NAD(P)+
-
 ?
1.1.1.79 glyoxylate + NADH + H+
-
 Spinacia oleracea glycolate + NAD+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.79 tetramer 4 * 33000, SDS-PAGE Spinacia oleracea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.79 6.5 7.4
-
 Spinacia oleracea

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.79 NAD(P)H
-
 Triticum aestivum
1.1.1.79 NAD(P)H
-
 Spinacia oleracea
1.1.1.79 NAD(P)H
-
 Glycine max