EC Number | General Stability | Organism |
---|---|---|
1.1.1.75 | dialysis overnight at 4°C, no loss of activity | Pseudomonas sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.75 | (R)-1-aminopropan-2-ol | competitive | Pseudomonas sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.75 | 0.12 | - |
D,L-1-aminopropan-2-ol | - |
Pseudomonas sp. | |
1.1.1.75 | 0.2 | - |
NADP+ | with D,L-1-aminopropan-2-ol as substrate | Pseudomonas sp. | |
1.1.1.75 | 0.3 | - |
NAD+ | with D,L-1-aminopropan-2-ol as substrate | Pseudomonas sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.75 | 70000 | 80000 | gel filtration | Pseudomonas sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.75 | L-1-aminopropan-2-ol + NAD+ | Pseudomonas sp. | involved in L-threonine and vitamin B12 pathway | aminoacetone + NADH | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.75 | Pseudomonas sp. | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.75 | partial | Pseudomonas sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.75 | 0.001 | - |
with 1-aminopropanol as growth substrate | Pseudomonas sp. |
1.1.1.75 | 0.4 | - |
crude extract | Pseudomonas sp. |
1.1.1.75 | 8.5 | - |
purified enzyme | Pseudomonas sp. |
EC Number | Storage Stability | Organism |
---|---|---|
1.1.1.75 | -15°C phosphate buffer, pH 7.2, 1 mg protein/ml, stable for more than 1 year | Pseudomonas sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.75 | 1,3-diaminopropan-2-ol + NAD+ | poor substrate | Pseudomonas sp. | 2-aminoacetamide + NADH | - |
? | |
1.1.1.75 | D,L-1-aminopropan-2-ol + NAD+ | - |
Pseudomonas sp. | aminoacetone + NADH | - |
? | |
1.1.1.75 | D,L-2-hydroxy-2-phenylethylamine + NAD+ | - |
Pseudomonas sp. | 2-amino-1-phenylethanone + NADH | - |
? | |
1.1.1.75 | D,L-3-hydroxybutyrate + NAD+ | - |
Pseudomonas sp. | ? | - |
? | |
1.1.1.75 | D,L-aminobutan-2-ol + NAD+ | - |
Pseudomonas sp. | 1-amino-2-oxobutane + NADH | - |
? | |
1.1.1.75 | D,L-phenylserine + NAD+ | - |
Pseudomonas sp. | ? | - |
? | |
1.1.1.75 | L-1-aminopropan-2-ol + NAD+ | no activity with D-enantiomer | Pseudomonas sp. | aminoacetone + NADH | - |
r | |
1.1.1.75 | L-1-aminopropan-2-ol + NAD+ | involved in L-threonine and vitamin B12 pathway | Pseudomonas sp. | aminoacetone + NADH | - |
r | |
1.1.1.75 | L-1-aminopropan-2-ol + NADP+ | about the same activity as with NAD+ as cofactor | Pseudomonas sp. | aminoacetone + NADPH | - |
r |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.75 | 9.5 | - |
- |
Pseudomonas sp. |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.75 | 6 | 10.5 | low activity at pH 6, 80% of activity at pH 10.5 | Pseudomonas sp. |