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Literature summary extracted from

  • Rosengarten, E.; Aman, P.; Thelin, S.; Hansson, C.; Ahlfors, S.; Bjoerk, P.; Jacobson, L.; Rorsman, H.
    The macrophage inhibitory factor MIF is a phenylpyruvate tautomerase (1997), FEBS Lett., 417, 85-88.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.2.1 expression in Escherichia coli, via pKK223-3 expression vector Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.2.1 2.4
-
 (4-hydroxyphenyl)pyruvate
-
 Homo sapiens
5.3.2.1 6
-
 phenylpyruvate
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.2.1 12000
-
 x * 12000, SDS-PAGE Homo sapiens
5.3.2.1 12000
-
 x * 12000, SDS-PAGE Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.2.1 (4-Hydroxyphenyl)pyruvate Homo sapiens
-
 ?
-
 ?
5.3.2.1 Phenylpyruvate Homo sapiens
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.3.2.1 Bos taurus
-
-
-
5.3.2.1 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.3.2.1 lens
-
 Bos taurus
-
5.3.2.1 liver
-
 Homo sapiens
-
5.3.2.1 liver
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.2.1 (4-Hydroxyphenyl)pyruvate
-
 Homo sapiens ?
-
 ?
5.3.2.1 3-(4-hydroxyphenyl)pyruvate keto-form Homo sapiens 2-hydroxy-3-(4-hydroxyphenyl)prop-2-enoate
-
 ?
5.3.2.1 3-(4-hydroxyphenyl)pyruvate keto-form Bos taurus 2-hydroxy-3-(4-hydroxyphenyl)prop-2-enoate
-
 ?
5.3.2.1 Phenylpyruvate keto-form Homo sapiens Phenylpyruvate
-
 ?
5.3.2.1 Phenylpyruvate keto-form Bos taurus Phenylpyruvate
-
 ?
5.3.2.1 Phenylpyruvate
-
 Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.2.1 ? x * 12000, SDS-PAGE Homo sapiens
5.3.2.1 ? x * 12000, SDS-PAGE Bos taurus