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Literature summary extracted from

  • Hatanaka, A.; Kajiwara, T.; Tomohiro, S.
    Purification and properties of alcohol dehydrogenase from Leuconostoc mesenteroides (1974), Agric. Biol. Chem., 38, 1819-1833.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.71 4-chloromercuribenzoate 1 mM, 100% inhibition Leuconostoc mesenteroides
1.1.1.71 8-hydroxyquinoline 1 mM, 100% inhibition with NAD+ as cofactor, 40% with NADP+ as cofactor Leuconostoc mesenteroides
1.1.1.71 AgNO3 1 mM, 100% inhibition Leuconostoc mesenteroides
1.1.1.71 CdCl2 1 mM, 24% inhibition with NAD+ as cofactor Leuconostoc mesenteroides
1.1.1.71 CuSO4 1 mM, 75% inhibition with NAD+ as cofactor, 56% with NADP+ as cofactor Leuconostoc mesenteroides
1.1.1.71 FeSO4 1 mM, 75% inhibition with NAD+ as cofactor, 46% with NADP+ as cofactor Leuconostoc mesenteroides
1.1.1.71 HgCl2 1 mM, 100% inhibition Leuconostoc mesenteroides
1.1.1.71 iodoacetic acid 1 mM, 29% inhibition with NAD+ as cofactor, 80% with NADP+ as cofactor Leuconostoc mesenteroides
1.1.1.71 o-phenanthroline 1 mM, 100% inhibition with NAD+ as cofactor, 40% with NADP+ as cofactor Leuconostoc mesenteroides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.71 0.059
-
 NADP+ oxidation of ethanol Leuconostoc mesenteroides
1.1.1.71 0.071
-
 NADPH reduction of acrylaldehyde Leuconostoc mesenteroides
1.1.1.71 0.072
-
 NADPH reduction of acetaldehyde Leuconostoc mesenteroides
1.1.1.71 0.095
-
 NADH reduction of acetaldehyde Leuconostoc mesenteroides
1.1.1.71 0.125
-
 NADP+ oxidation of allyl alcohol Leuconostoc mesenteroides
1.1.1.71 0.22
-
 NADH reduction of acrylaldehyde Leuconostoc mesenteroides
1.1.1.71 0.5
-
 NAD+ oxidation of allyl alcohol Leuconostoc mesenteroides
1.1.1.71 0.83
-
 NAD+ oxidation of ethanol Leuconostoc mesenteroides

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.71 Leuconostoc mesenteroides
-
-
-
1.1.1.71 Leuconostoc mesenteroides IFO 3426
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.71 ammonium sulfate, DEAE-Sephadex A-50, hydroxyapatite, Sephadex G-200 Leuconostoc mesenteroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.71 allyl alcohol + NADP+
-
 Leuconostoc mesenteroides acrylaldehyde + NADPH
-
 r
1.1.1.71 allyl alcohol + NADP+
-
 Leuconostoc mesenteroides IFO 3426 acrylaldehyde + NADPH
-
 r
1.1.1.71 butan-1-ol + NADP+
-
 Leuconostoc mesenteroides butanal + NADPH
-
 r
1.1.1.71 butan-1-ol + NADP+
-
 Leuconostoc mesenteroides IFO 3426 butanal + NADPH
-
 r
1.1.1.71 cis-2-butenol + NAD(P)H
-
 Leuconostoc mesenteroides trans-2-butenal + NAD(P)+
-
 ?
1.1.1.71 cis-2-butenol + NAD(P)H
-
 Leuconostoc mesenteroides IFO 3426 trans-2-butenal + NAD(P)+
-
 ?
1.1.1.71 cis-4-heptenal + NAD(P)+
-
 Leuconostoc mesenteroides cis-4-heptenol + NAD(P)H
-
 ?
1.1.1.71 ethanol + NADP+
-
 Leuconostoc mesenteroides ethanal + NADPH + H+
-
 r
1.1.1.71 ethanol + NADP+
-
 Leuconostoc mesenteroides IFO 3426 ethanal + NADPH + H+
-
 r
1.1.1.71 pentan-1-ol + NADP+
-
 Leuconostoc mesenteroides pentanal + NADPH
-
 r
1.1.1.71 pentan-1-ol + NADP+
-
 Leuconostoc mesenteroides IFO 3426 pentanal + NADPH
-
 r
1.1.1.71 retinaldehyde + NADH
-
 Leuconostoc mesenteroides retinol + NAD+
-
 ?
1.1.1.71 trans-2,cis-6-nonadienal + NAD(P)+
-
 Leuconostoc mesenteroides trans-2,cis-6-nonadienol + NAD(P)H
-
 ?
1.1.1.71 trans-2-pentenol + NAD(P)+
-
 Leuconostoc mesenteroides trans-2-pentenal + NAD(P)H
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.71 30
-
 no loss of activity after 15 min, complete loss of activity at 60°C Leuconostoc mesenteroides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.71 8.6
-
 cofactor NADP+ Leuconostoc mesenteroides
1.1.1.71 10.2
-
 cofactor NAD+ Leuconostoc mesenteroides

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.71 8 9.8
-
 Leuconostoc mesenteroides

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.71 NAD(P)H
-
 Leuconostoc mesenteroides