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Literature summary extracted from
- Kinetic study of the catalytic mechanism of mannitol dehydrogenase from Pseudomonas fluorescens (1999), Biochemistry, 38, 10489-10498.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.1.1.67 | expression in Escherichia coli | Pseudomonas fluorescens |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.67 | 1.2 | - |
D-mannitol | recombinant protein | Pseudomonas fluorescens |  |
| 1.1.1.67 | 25 | - |
D-fructose | recombinant protein | Pseudomonas fluorescens | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.67 | 45000 | - |
recombinant protein, gel filtration | Pseudomonas fluorescens |
| 1.1.1.67 | 54000 | - |
1 * 54000, SDS-PAGE, recombinant protein | Pseudomonas fluorescens |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.67 | Pseudomonas fluorescens | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.67 | recombinant protein | Pseudomonas fluorescens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.67 | D-mannitol + NAD+ | - |
Pseudomonas fluorescens | D-fructose + NADH + H+ | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.1.67 | monomer | 1 * 54000, SDS-PAGE, recombinant protein | Pseudomonas fluorescens |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.1.67 | MDH | - |
Pseudomonas fluorescens |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.67 | 20 | - |
NAD+ | recombinant protein | Pseudomonas fluorescens | |
| 1.1.1.67 | 54 | - |
NADH | recombinant protein | Pseudomonas fluorescens | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.67 | 7 | - |
D-fructose reduction, recombinant protein | Pseudomonas fluorescens |
| 1.1.1.67 | 10 | - |
D-mannitol oxidation, recombinant protein | Pseudomonas fluorescens |
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Release 2023.1 (January 2023)
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