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Literature summary extracted from

  • Ueng, S.T.H.; Hartanowicz, P.; Lewandowski, C.; Keller, J.; M.Holick, E.T.McGuinness
    D-Mannitol dehydrogenase from Absidia glauca. Purification, metabolic role, and subunit interactions (1976), Biochemistry, 15, 1743-1749.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.67 Methyl mercurinitrate complete inhibition Absidia glauca
1.1.1.67 p-hydroxymercuribenzoate complete inhibition Absidia glauca

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.67 67000
-
 1 * 67000 Absidia glauca
1.1.1.67 133000
-
 gel filtration Absidia glauca

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.67 D-mannitol + NAD+ Absidia glauca
-
 D-fructose + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.67 Absidia glauca
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.67
-
 Absidia glauca

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.67 10.24
-
-
 Absidia glauca

Storage Stability

EC Number Storage Stability Organism
1.1.1.67 -20°C, 10 mM dithiothreitol, 45 days Absidia glauca

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.67 D-arabinitol + NAD+
-
 Absidia glauca D-ribulose + NADH + H+
-
 ?
1.1.1.67 D-mannitol + NAD+
-
 Absidia glauca D-fructose + NADH + H+
-
 ?
1.1.1.67 D-mannitol + NAD+
-
 Absidia glauca D-fructose + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.67 monomer 1 * 67000 Absidia glauca

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.67 20
-
 not stable above Absidia glauca

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.67 6 7.2 D-fructose reduction Absidia glauca
1.1.1.67 8.9 10 D-mannitol oxidation Absidia glauca

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.67 6 6.5 not active below, D-mannitol oxidation Absidia glauca

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.67 NAD+
-
 Absidia glauca
1.1.1.67 NADH
-
 Absidia glauca