Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Pollow, K.; Luebbert, H.; Pollow, B.
    On the mitochondrial 17beta-hydroxysteroid dehydrogenase from human endometrium and endometrial carcinoma: characterization and intramitochondrial distribution (1976), J. Steroid Biochem., 7, 45-50.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.62 Cu2+ 1 mM, 82% inhibition Homo sapiens
1.1.1.62 iodoacetamide 1 mM, 78% inhibition Homo sapiens
1.1.1.62 Mg2+ 1 mM, 18% inhibition Homo sapiens
1.1.1.62 PCMB 1 mM, complete inhibition Homo sapiens
1.1.1.62 Zn2+ 1 mM, 33% inhibition Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.62 0.0032
-
 estradiol-17beta cosubstrate NADP+, secretory endometrium Homo sapiens
1.1.1.62 0.0036
-
 estradiol cosubstrate NAD+, secretory endometrium Homo sapiens
1.1.1.62 0.0128
-
 estradiol-17beta cosubstrate NADP+, proliferating endometrium Homo sapiens
1.1.1.62 0.0134
-
 estradiol cosubstrate NAD+, proliferating endometrium Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.62 mitochondrial outer membrane
-
 Homo sapiens 5741
-

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.62 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.62
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.62 uterine endometrium proliferative Homo sapiens
-
1.1.1.62 uterine endometrium secretory Homo sapiens
-
1.1.1.62 uterine endometrium endometrial carcinoma Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.62 4-androstene-3,17-dione + NADPH + H+
-
 Homo sapiens testosterone + NADP+
-
 r
1.1.1.62 androstenedione + NADPH + H+ 50% of the activity with estradiol-17beta Homo sapiens testosterone + NADP+
-
 ?
1.1.1.62 estradiol-17beta + NAD+
-
 Homo sapiens estrone + NADH
-
 r
1.1.1.62 estradiol-17beta + NADP+
-
 Homo sapiens estrone + NADPH
-
 r
1.1.1.62 estrone + NADH
-
 Homo sapiens estradiol-17beta + NAD+
-
 r
1.1.1.62 estrone + NADPH + H+
-
 Homo sapiens estradiol-17beta + NADP+
-
 r
1.1.1.62 testosterone + NADP+ 75% of the activity with estradiol-17beta Homo sapiens 4-androstene-3,17-dione + NADPH + H+
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.62 45
-
 enzyme from crude washed mitochondria Homo sapiens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.62 24 54 24°C: about 60% of maximal activity, 54°C: about 50% of maximal activity, enzyme from crude washed mitochondria Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.62 6
-
 reduction of estrone Homo sapiens
1.1.1.62 9.5
-
 oxidation of estradiol-17beta Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.62 NAD+ cofactor Homo sapiens
1.1.1.62 NADH cofactor Homo sapiens
1.1.1.62 NADP+ cofactor Homo sapiens
1.1.1.62 NADPH cofactor Homo sapiens