Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kahle, C.; Schneider, K.H.; Giffhorn, F.
    Pentitol metabolism of Rodobacter sphaeroides Si4: purification and characterization of a ribitol dehydrogenase (1992), J. Gen. Microbiol., 138, 1277-1281.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.56 0.077
-
 NAD+ pH 9, 30°C Cereibacter sphaeroides
1.1.1.56 1.1
-
 D-ribulose pH 9, 30°C Cereibacter sphaeroides
1.1.1.56 6.3
-
 ribitol pH 9, 30°C Cereibacter sphaeroides
1.1.1.56 12.5
-
 D-xylulose pH 9, 30°C Cereibacter sphaeroides
1.1.1.56 77
-
 xylitol pH 9, 30°C Cereibacter sphaeroides

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.56 additional information
-
 Cereibacter sphaeroides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.56 25000
-
 2 * 25000, SDS-PAGE Cereibacter sphaeroides
1.1.1.56 50000
-
 gel filtration Cereibacter sphaeroides

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.56 Cereibacter sphaeroides
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.56
-
 Cereibacter sphaeroides

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.56 59
-
-
 Cereibacter sphaeroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.56 D-arabitol + NAD+
-
 Cereibacter sphaeroides D-xylulose + NADH + H+
-
 ?
1.1.1.56 D-glucitol + NAD+
-
 Cereibacter sphaeroides ? + NADH
-
 ?
1.1.1.56 erythritol + NAD+
-
 Cereibacter sphaeroides ? + NADH
-
 ?
1.1.1.56 ribitol + NAD+
-
 Cereibacter sphaeroides D-ribulose + NADH
-
 ?
1.1.1.56 xylitol + NAD+
-
 Cereibacter sphaeroides D-xylulose + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.56 dimer 2 * 25000, SDS-PAGE Cereibacter sphaeroides

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.56 45
-
-
 Cereibacter sphaeroides

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.56 4 60 50% enzyme activity loss after 1 min to 15 days Cereibacter sphaeroides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.56 6.5
-
 sugar reduction Cereibacter sphaeroides
1.1.1.56 9 10.3 alcohol oxidation Cereibacter sphaeroides

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.56 8
-
 50% of maximal activity Cereibacter sphaeroides
1.1.1.56 10.7
-
 50% of maximal activity Cereibacter sphaeroides

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.56 NAD+
-
 Cereibacter sphaeroides
1.1.1.56 NADH
-
 Cereibacter sphaeroides