Literature summary extracted from

Maurer, P.; Lessmann, D.; Kurz, G.
D-Glucose-6-phosphate dehydrogenases from Pseudomonas fluorescens (1982), Methods Enzymol., 89, 261-270.

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
1.1.1.49	55000	-	4 * 55000, Entner-Doudoroff enzyme	Pseudomonas fluorescens
1.1.1.49	65000	-	4 * 65000, Zwischenferment	Pseudomonas fluorescens
1.1.1.49	220000	-	Entner-Doudoroff enzyme, gel chromatography	Pseudomonas fluorescens
1.1.1.49	265000	-	Zwischenferment, gel chromatography	Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
1.1.1.49	D-glucose 6-phosphate + H2O + NADP+	Pseudomonas fluorescens	-	D-gluconate 6-phosphate + NADPH + H+	6-phosphoglucono-delta-lactone	r

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.1.1.49	Pseudomonas fluorescens	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.1.1.49	-	Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.1.1.49	additional information	-	-	Pseudomonas fluorescens

Storage Stability

EC Number	Storage Stability	Organism
1.1.1.49	4°C, several months	Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.1.1.49	D-glucose 6-phosphate + H2O + NADP+	-	Pseudomonas fluorescens	D-gluconate 6-phosphate + NADPH + H+	6-phosphoglucono-delta-lactone	r

Subunits

EC Number	Subunits	Comment	Organism
1.1.1.49	tetramer	4 * 65000, Zwischenferment	Pseudomonas fluorescens
1.1.1.49	tetramer	4 * 55000, Entner-Doudoroff enzyme	Pseudomonas fluorescens

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
1.1.1.49	30	-	assay at	Pseudomonas fluorescens

pH Stability

EC Number	pH Stability	pH Stability Maximum	Comment	Organism
1.1.1.49	6	10.5	stable at 5°C for 48 h	Pseudomonas fluorescens
1.1.1.49	6.5	10	Entner-Doudoroff enzyme stable	Pseudomonas fluorescens

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.1.1.49	NAD+	-	Pseudomonas fluorescens
1.1.1.49	NADP+	-	Pseudomonas fluorescens
1.1.1.49	NADPH	-	Pseudomonas fluorescens

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Release 2023.1 (January 2023)