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Literature summary extracted from

  • Kato, N.; Sahm, H.; Schutte, H.; Wagner, F.
    Purification and properties of glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase from a methanol-utilizing yeast, Candida boidinii (1979), Biochim. Biophys. Acta, 566, 1-11.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.44 ATP
-
 [Candida] boidinii
1.1.1.44 ATP
-
 Ovis aries
1.1.1.44 D-fructose 1,6-diphosphate
-
 [Candida] boidinii
1.1.1.44 D-fructose 1,6-diphosphate
-
 Ovis aries
1.1.1.44 D-ribulose 5-phosphate
-
 [Candida] boidinii
1.1.1.44 NADPH competitive inhibition [Candida] boidinii
1.1.1.44 UTP
-
 [Candida] boidinii
1.1.1.44 UTP
-
 Ovis aries
1.1.1.49 AMP
-
 [Candida] boidinii
1.1.1.49 glyceraldehyde 3-phosphate
-
 [Candida] boidinii
1.1.1.49 GTP
-
 [Candida] boidinii
1.1.1.49 NADPH
-
 [Candida] boidinii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.44 0.011
-
 6-phosphogluconate
-
 [Candida] boidinii
1.1.1.44 0.013
-
 NADP+
-
 [Candida] boidinii
1.1.1.49 additional information
-
 additional information
-
 [Candida] boidinii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.44 58000
-
 2 * 58000, SDS-PAGE [Candida] boidinii
1.1.1.44 110000
-
 gel filtration [Candida] boidinii
1.1.1.49 61000
-
 2 * 61000, SDS gel electrophoresis [Candida] boidinii
1.1.1.49 118000 120000 gel filtration [Candida] boidinii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.44 6-phospho-D-gluconate + NADP+ Saccharomyces cerevisiae
-
 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
1.1.1.44 6-phospho-D-gluconate + NADP+ Ovis aries
-
 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
1.1.1.44 6-phospho-D-gluconate + NADP+ [Candida] boidinii
-
 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
1.1.1.49 D-glucose 6-phosphate + H2O + NADP+ [Candida] boidinii
-
 D-gluconate 6-phosphate + NADPH + H+ 6-phosphoglucono-delta-lactone r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.44 Ovis aries
-
 sheep
-
1.1.1.44 Saccharomyces cerevisiae
-
-
-
1.1.1.44 [Candida] boidinii
-
 methanol utilizing yeast
-
1.1.1.44 [Candida] boidinii
-
 ATCC 32195
-
1.1.1.49 [Candida] boidinii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.44
-
 [Candida] boidinii
1.1.1.49
-
 [Candida] boidinii

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.44 liver
-
 Ovis aries
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.44 39.7
-
-
 [Candida] boidinii

Storage Stability

EC Number Storage Stability Organism
1.1.1.49 5°C, pH 6.0-10.5, stable for 48 h [Candida] boidinii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.44 6-phospho-D-gluconate + NADP+
-
 Saccharomyces cerevisiae D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
1.1.1.44 6-phospho-D-gluconate + NADP+
-
 Ovis aries D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
1.1.1.44 6-phospho-D-gluconate + NADP+
-
 [Candida] boidinii D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
1.1.1.49 D-glucose 6-phosphate + H2O + NADP+
-
 [Candida] boidinii D-gluconate 6-phosphate + NADPH + H+ 6-phosphoglucono-delta-lactone r

Subunits

EC Number Subunits Comment Organism
1.1.1.44 dimer
-
 Ovis aries
1.1.1.44 dimer 2 * 58000, SDS-PAGE [Candida] boidinii
1.1.1.49 dimer 2 * 61000, SDS gel electrophoresis [Candida] boidinii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.49 55
-
-
 [Candida] boidinii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.49 5
-
 48 h [Candida] boidinii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.49 8 9
-
 [Candida] boidinii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.49 6 10.5 stable at 5°C for 48 h [Candida] boidinii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.49 NADP+
-
 [Candida] boidinii
1.1.1.49 NADPH
-
 [Candida] boidinii