Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Carlsson, H.; Ljung, S.; Bulow, L.
    Physical and kinetic effects on introduction of various linker regions in beta-galactosidase/galactose dehydrogenase fusion enzymes (1996), Biochim. Biophys. Acta, 1293, 154-160.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.48 beta-galactosidase-galactose dehydrogenase fusion enzymes, expressed in Escherichia coli Pseudomonas fluorescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.48 0.15
-
 NAD+
-
 Pseudomonas fluorescens
1.1.1.48 0.23
-
 D-galactose
-
 Pseudomonas fluorescens
1.1.1.48 0.25
-
 NAD+ beta-galactosidase-galactose dehydrogenase fusion enzymes Pseudomonas fluorescens
1.1.1.48 0.37
-
 D-galactose beta-galactosidase-galactose dehydrogenase fusion enzyme, 13 amino acids as linker Pseudomonas fluorescens
1.1.1.48 0.4
-
 D-galactose beta-galactosidase-galactose dehydrogenase fusion enzyme, 9 amino acids as linker Pseudomonas fluorescens
1.1.1.48 0.45
-
 D-galactose beta-galactosidase-galactose dehydrogenase fusion enzyme, 3 amino acids as linker Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.48 D-galactose + NAD+ Pseudomonas fluorescens
-
 D-galactono-1,5-lactone + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.48 Pseudomonas fluorescens
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.48 17
-
 beta-galactosidase-galactose dehydrogenase fusion enzyme, 3 amino acids as linker Pseudomonas fluorescens
1.1.1.48 27
-
 beta-galactosidase-galactose dehydrogenase fusion enzyme, 13 amino acids as linker Pseudomonas fluorescens
1.1.1.48 29
-
 beta-galactosidase-galactose dehydrogenase fusion enzyme, 9 amino acids as linker Pseudomonas fluorescens
1.1.1.48 34
-
-
 Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.48 D-galactose + NAD+
-
 Pseudomonas fluorescens D-galactono-1,5-lactone + NADH
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.48 50
-
 native enzyme, 70% activity after 20 min, 30% after 60 min, 20% after 90 min, beta-galactosidase-galactose dehydrogenase fusion enzymes, 80% activity after 90 min Pseudomonas fluorescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.48 9.9
-
-
 Pseudomonas fluorescens