EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.1.1.44 | - |
Geobacillus stearothermophilus |
EC Number | General Stability | Organism |
---|---|---|
1.1.1.44 | noteworthy stability to heat and protein denaturants | Geobacillus stearothermophilus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.44 | 5,5'-dithiobis(2-nitrobenzoic acid) | - |
Cyberlindnera jadinii | |
1.1.1.44 | 5,5'-dithiobis(2-nitrobenzoic acid) | - |
Escherichia coli | |
1.1.1.44 | 5,5'-dithiobis(2-nitrobenzoic acid) | Ellman's reagent | Geobacillus stearothermophilus | |
1.1.1.44 | 7-Chloro-4-nitrobenzo-2-oxa-1,3-diazole | NBD-Cl | Geobacillus stearothermophilus | |
1.1.1.44 | iodoacetamide | - |
Geobacillus stearothermophilus | |
1.1.1.44 | p-hydroxymercuribenzoate | - |
Geobacillus stearothermophilus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.44 | Mg2+ | - |
Cyberlindnera jadinii | |
1.1.1.44 | Mg2+ | - |
Geobacillus stearothermophilus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.44 | 50000 | - |
2 * 50000, SDS-PAGE | Geobacillus stearothermophilus |
1.1.1.44 | 100000 | - |
- |
Cyberlindnera jadinii |
1.1.1.44 | 100000 | - |
- |
Escherichia coli |
1.1.1.44 | 100000 | - |
sucrose density gradient centrifugation | Geobacillus stearothermophilus |
1.1.1.44 | 100000 | - |
sedimentation equilibrium | Geobacillus stearothermophilus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.44 | 6-phospho-D-gluconate + NADP+ | Cyberlindnera jadinii | - |
D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
1.1.1.44 | 6-phospho-D-gluconate + NADP+ | Escherichia coli | enzyme specific for the oxidative pentose phosphate pathway | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
1.1.1.44 | 6-phospho-D-gluconate + NADP+ | Geobacillus stearothermophilus | enzyme specific for the oxidative pentose phosphate pathway | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
1.1.1.44 | 6-phospho-D-gluconate + NADP+ | Geobacillus stearothermophilus NCA 1503 | enzyme specific for the oxidative pentose phosphate pathway | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.44 | Cyberlindnera jadinii | - |
- |
- |
1.1.1.44 | Escherichia coli | - |
- |
- |
1.1.1.44 | Geobacillus stearothermophilus | - |
NCA 1503 | - |
1.1.1.44 | Geobacillus stearothermophilus NCA 1503 | - |
NCA 1503 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.44 | - |
Geobacillus stearothermophilus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.44 | 27 | - |
- |
Geobacillus stearothermophilus |
EC Number | Storage Stability | Organism |
---|---|---|
1.1.1.44 | 4°C, retains full activity for several months when stored as an ammonium sulfate suspension or in acetate buffer, pH 6 in presence of a crystal of thymol to prevent bacterial growth | Geobacillus stearothermophilus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.44 | 6-phospho-D-gluconate + NADP+ | - |
Cyberlindnera jadinii | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
1.1.1.44 | 6-phospho-D-gluconate + NADP+ | - |
Escherichia coli | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
1.1.1.44 | 6-phospho-D-gluconate + NADP+ | - |
Geobacillus stearothermophilus | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
1.1.1.44 | 6-phospho-D-gluconate + NADP+ | enzyme specific for the oxidative pentose phosphate pathway | Escherichia coli | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
1.1.1.44 | 6-phospho-D-gluconate + NADP+ | enzyme specific for the oxidative pentose phosphate pathway | Geobacillus stearothermophilus | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
1.1.1.44 | 6-phospho-D-gluconate + NADP+ | - |
Geobacillus stearothermophilus NCA 1503 | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
1.1.1.44 | 6-phospho-D-gluconate + NADP+ | enzyme specific for the oxidative pentose phosphate pathway | Geobacillus stearothermophilus NCA 1503 | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.44 | dimer | 2 * 50000, SDS-PAGE | Geobacillus stearothermophilus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.44 | 25 | - |
maximum activity at pH 8 also at 43°C | Geobacillus stearothermophilus |
1.1.1.44 | 43 | - |
maximum activity at pH 8 also at 25°C | Geobacillus stearothermophilus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.44 | 50 | 60 | - |
Escherichia coli |
1.1.1.44 | 65 | 75 | - |
Geobacillus stearothermophilus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.44 | 60 | - |
retains full activity after heating for 15 min | Geobacillus stearothermophilus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.44 | 8 | - |
- |
Cyberlindnera jadinii |
1.1.1.44 | 8 | - |
- |
Geobacillus stearothermophilus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.44 | 6 | 9 | - |
Geobacillus stearothermophilus |