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Literature summary extracted from
- Isolation and properties of 6-phosphogluconate dehydrogenase from Escherichia coli. Some Comparisons with the thermophilic enzyme from Bacillus stearothermophilus (1976), Biochemistry, 15, 4026-4033.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.1.1.44 | denaturation by urea | Escherichia coli |
| 1.1.1.44 | stable in 8 M urea, nearly unaffected, only 10% loss of activity after exposure for 60 min | Geobacillus stearothermophilus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.44 | 5,5'-dithiobis(2-nitrobenzoic acid) | - |
Cyberlindnera jadinii |  |
| 1.1.1.44 | 5,5'-dithiobis(2-nitrobenzoic acid) | - |
Enterococcus faecalis | |
| 1.1.1.44 | 5,5'-dithiobis(2-nitrobenzoic acid) | - |
Escherichia coli | |
| 1.1.1.44 | 5,5'-dithiobis(2-nitrobenzoic acid) | - |
Geobacillus stearothermophilus | |
| 1.1.1.44 | D-fructose 1,6-diphosphate | - |
Enterococcus faecalis | |
| 1.1.1.44 | D-fructose 1,6-diphosphate | - |
Escherichia coli | |
| 1.1.1.44 | D-fructose 1,6-diphosphate | - |
Neurospora crassa | |
| 1.1.1.44 | NADPH | - |
Escherichia coli | |
| 1.1.1.44 | NADPH | - |
Geobacillus stearothermophilus | |
| 1.1.1.44 | NADPH | - |
Neurospora crassa | |
| 1.1.1.44 | p-chloromercuribenzoate | - |
Enterococcus faecalis | |
| 1.1.1.44 | p-chloromercuribenzoate | - |
Escherichia coli | |
| 1.1.1.44 | p-chloromercuribenzoate | - |
Geobacillus stearothermophilus | |
| 1.1.1.44 | Rose bengal | photooxidation | Escherichia coli | |
| 1.1.1.44 | Rose bengal | photooxidation | Geobacillus stearothermophilus | |
| 1.1.1.44 | Rose bengal | photooxidation | Neurospora crassa | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.44 | 0.033 | - |
NADP+ | - |
Geobacillus stearothermophilus | |
| 1.1.1.44 | 0.05 | - |
6-phosphogluconate | - |
Geobacillus stearothermophilus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.44 | 100000 | - |
gel filtration | Cyberlindnera jadinii |
| 1.1.1.44 | 100000 | - |
gel filtration | Escherichia coli |
| 1.1.1.44 | 100000 | - |
sucrose density gradient centrifugation | Geobacillus stearothermophilus |
| 1.1.1.44 | 106000 | 115000 | - |
Enterococcus faecalis |
| 1.1.1.44 | 110000 | 120000 | - |
Neurospora crassa |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.44 | 6-phospho-D-gluconate + NADP+ | Cyberlindnera jadinii | - |
D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
| 1.1.1.44 | 6-phospho-D-gluconate + NADP+ | Enterococcus faecalis | - |
D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
| 1.1.1.44 | 6-phospho-D-gluconate + NADP+ | Escherichia coli | enzyme specific for the oxidative pentose phosphate pathway | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
| 1.1.1.44 | 6-phospho-D-gluconate + NADP+ | Geobacillus stearothermophilus | enzyme specific for the oxidative pentose phosphate pathway | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
| 1.1.1.44 | 6-phospho-D-gluconate + NADP+ | Neurospora crassa | enzyme specific for the oxidative pentose phosphate pathway | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
| 1.1.1.44 | 6-phospho-D-gluconate + NADP+ | Escherichia coli MRE 600 | enzyme specific for the oxidative pentose phosphate pathway | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.44 | Cyberlindnera jadinii | - |
- |
- |
| 1.1.1.44 | Enterococcus faecalis | - |
- |
- |
| 1.1.1.44 | Escherichia coli | - |
MRE 600 | - |
| 1.1.1.44 | Escherichia coli MRE 600 | - |
MRE 600 | - |
| 1.1.1.44 | Geobacillus stearothermophilus | - |
- |
- |
| 1.1.1.44 | Neurospora crassa | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.44 | - |
Escherichia coli |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.44 | 32 | - |
- |
Escherichia coli |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.1.1.44 | 20°C, in phosphate buffer pH 6.8 in presence of the reducing agent beta-mercaptoethanol enzyme remains fully active for 24 h | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.44 | 6-phospho-D-gluconate + NADP+ | - |
Cyberlindnera jadinii | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
| 1.1.1.44 | 6-phospho-D-gluconate + NADP+ | - |
Escherichia coli | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
| 1.1.1.44 | 6-phospho-D-gluconate + NADP+ | - |
Geobacillus stearothermophilus | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
| 1.1.1.44 | 6-phospho-D-gluconate + NADP+ | - |
Neurospora crassa | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
| 1.1.1.44 | 6-phospho-D-gluconate + NADP+ | - |
Enterococcus faecalis | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
| 1.1.1.44 | 6-phospho-D-gluconate + NADP+ | enzyme specific for the oxidative pentose phosphate pathway | Escherichia coli | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
| 1.1.1.44 | 6-phospho-D-gluconate + NADP+ | enzyme specific for the oxidative pentose phosphate pathway | Geobacillus stearothermophilus | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
| 1.1.1.44 | 6-phospho-D-gluconate + NADP+ | enzyme specific for the oxidative pentose phosphate pathway | Neurospora crassa | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
| 1.1.1.44 | 6-phospho-D-gluconate + NADP+ | - |
Escherichia coli MRE 600 | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
| 1.1.1.44 | 6-phospho-D-gluconate + NADP+ | enzyme specific for the oxidative pentose phosphate pathway | Escherichia coli MRE 600 | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.1.44 | dimer | - |
Cyberlindnera jadinii |
| 1.1.1.44 | dimer | - |
Escherichia coli |
| 1.1.1.44 | dimer | - |
Geobacillus stearothermophilus |
| 1.1.1.44 | dimer | - |
Neurospora crassa |
| 1.1.1.44 | dimer | - |
Enterococcus faecalis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.44 | 55 | 60 | - |
Escherichia coli |
| 1.1.1.44 | 75 | 80 | - |
Geobacillus stearothermophilus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.44 | 45 | - |
stable below | Escherichia coli |
| 1.1.1.44 | 60 | - |
retains more than 90% of its structure | Geobacillus stearothermophilus |
| 1.1.1.44 | 67 | - |
stable below | Geobacillus stearothermophilus |
| 1.1.1.44 | 70 | - |
stable up to | Geobacillus stearothermophilus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.44 | NADP+ | - |
Cyberlindnera jadinii | |
| 1.1.1.44 | NADP+ | - |
Escherichia coli | |
| 1.1.1.44 | NADP+ | - |
Neurospora crassa | |
| 1.1.1.44 | NADP+ | - |
Enterococcus faecalis | |
| 1.1.1.44 | NADP+ | strictly specific for NADP+, no activity observed with NAD+ | Geobacillus stearothermophilus | |
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