EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.43 | adenosine 5'-triphosphate | ATP inhibits the enzyme which is active with NAD+, not the NADP+ dependent activity | Burkholderia cepacia | |
1.1.1.43 | ATP | NAD+-specific enzyme | Enterococcus faecalis | |
1.1.1.43 | fructose 1,6-bisphosphate | NADP+-specific enzyme | Enterococcus faecalis | |
1.1.1.43 | guanosine 5'-triphosphate | GTP inhibits the NADP+ specific enzyme | Burkholderia cepacia | |
1.1.1.43 | NADPH | inhibition is competitive with respect to NADP+ | Burkholderia cepacia |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.43 | 0.02 | - |
6-phospho-D-gluconate | - |
Burkholderia cepacia | |
1.1.1.43 | 0.02 | - |
NADP+ | enzyme active with NAD+ and NADP+-specific enzyme | Burkholderia cepacia | |
1.1.1.43 | 0.2 | - |
NAD+ | - |
Burkholderia cepacia | |
1.1.1.43 | 0.2 | - |
6-phospho-D-gluconate | - |
Burkholderia cepacia | |
1.1.1.43 | 0.3 | - |
6-phospho-D-gluconate | enzyme active with NAD+ or NADP+ | Burkholderia cepacia | |
1.1.1.43 | 0.7 | - |
6-phospho-D-gluconate | - |
Burkholderia cepacia | |
1.1.1.43 | 1 | - |
6-phospho-D-gluconate | 0.005 M GTP | Burkholderia cepacia |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.43 | 40000 | - |
2 * 40000, enzyme active with NAD+ | Burkholderia cepacia |
1.1.1.43 | 57000 | - |
2 * 57000 | Neurospora crassa |
1.1.1.43 | 60000 | - |
2 * 60000, enzyme specific for NADP+ | Burkholderia cepacia |
1.1.1.43 | 82000 | - |
enzyme active with NAD+, sucrose density gradient sedimentation | Burkholderia cepacia |
1.1.1.43 | 84000 | - |
enzyme active with NAD+, gel filtration | Burkholderia cepacia |
1.1.1.43 | 110000 | 120000 | NADP+-specific enzyme | Neurospora crassa |
1.1.1.43 | 120000 | - |
NADP+-specific enzyme, gel filtration | Burkholderia cepacia |
1.1.1.43 | 123000 | - |
NADP+-specific enzyme, sucrose density gradient sedimentation | Burkholderia cepacia |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.43 | 6-phospho-D-gluconate + NAD(P)+ | Neurospora crassa | - |
6-phospho-2-dehydro-D-gluconate + NAD(P)H | - |
r | |
1.1.1.43 | 6-phospho-D-gluconate + NAD(P)+ | Pseudomonas aeruginosa | - |
6-phospho-2-dehydro-D-gluconate + NAD(P)H | - |
r | |
1.1.1.43 | 6-phospho-D-gluconate + NAD(P)+ | Enterococcus faecalis | - |
6-phospho-2-dehydro-D-gluconate + NAD(P)H | - |
r | |
1.1.1.43 | 6-phospho-D-gluconate + NAD(P)+ | Burkholderia cepacia | - |
6-phospho-2-dehydro-D-gluconate + NAD(P)H | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.43 | Burkholderia cepacia | - |
ATCC 17616, synonymous with Pseudomonas cepacia | - |
1.1.1.43 | Enterococcus faecalis | - |
- |
- |
1.1.1.43 | Neurospora crassa | - |
- |
- |
1.1.1.43 | Pseudomonas aeruginosa | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.43 | - |
Burkholderia cepacia |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.43 | 34 | - |
NADP+ as cofactor | Burkholderia cepacia |
1.1.1.43 | 42.3 | - |
NAD+ as cofactor | Burkholderia cepacia |
EC Number | Storage Stability | Organism |
---|---|---|
1.1.1.43 | 0°C both 6-phosphogluconate dehydrogenase species obtained by treatment with ammonium sulfate retain 100% of their activity when stored for several months | Burkholderia cepacia |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.43 | 6-phospho-D-gluconate + NAD(P)+ | - |
Neurospora crassa | 6-phospho-2-dehydro-D-gluconate + NAD(P)H | - |
r | |
1.1.1.43 | 6-phospho-D-gluconate + NAD(P)+ | - |
Pseudomonas aeruginosa | 6-phospho-2-dehydro-D-gluconate + NAD(P)H | - |
r | |
1.1.1.43 | 6-phospho-D-gluconate + NAD(P)+ | - |
Enterococcus faecalis | 6-phospho-2-dehydro-D-gluconate + NAD(P)H | - |
r | |
1.1.1.43 | 6-phospho-D-gluconate + NAD(P)+ | - |
Burkholderia cepacia | 6-phospho-2-dehydro-D-gluconate + NAD(P)H | - |
r | |
1.1.1.43 | D-glucose-6-phosphate + NAD+ | enzyme active with NAD+, 2% of the activity compared to 6-phosphogluconate when NAD+ is cofactor, no activity detected with NADP+ as cofactor | Burkholderia cepacia | 2-dehydro-6-phospho-D-glucose + NADH | - |
? | |
1.1.1.43 | D-glucose-6-phosphate + NADP+ | NADP-specific enzyme, 4% of the activity with 6-phosphogluconate | Burkholderia cepacia | 2-dehydro-6-phospho-D-glucose + NADPH | - |
? | |
1.1.1.43 | additional information | appears to lack 6PGAD except during growth on glucose or gluconate | Pseudomonas aeruginosa | ? | - |
? | |
1.1.1.43 | additional information | glucose, 2-deoxyglucose, gluconate, fructose-6-phosphate, glucose-1-phosphate, fructose-1,6-diphosphate, ribulose-5-phosphate and 1,3-diphosphoglycerate are no substrates | Burkholderia cepacia | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.43 | dimer | 2 * 40000, enzyme active with NAD+ | Burkholderia cepacia |
1.1.1.43 | dimer | 2 * 60000, enzyme specific for NADP+ | Burkholderia cepacia |
1.1.1.43 | dimer | 2 * 57000 | Neurospora crassa |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.43 | 6PGAD | - |
Pseudomonas aeruginosa |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.43 | 8.5 | - |
NADP-specific enzyme | Burkholderia cepacia |
1.1.1.43 | 8.5 | 9.5 | enzyme active with NAD+ | Burkholderia cepacia |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.43 | NAD+ | - |
Burkholderia cepacia |