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Literature summary extracted from

  • Ramaley, R.F.; Hudock, M.O.
    Purification and properties of isocitrate dehydrogenase (NADP) from Thermus aquaticus YT-1, Bacillus subtilis-168 and Chlamydomonas reinhardti-Y-2 (1973), Biochim. Biophys. Acta, 315, 22-36.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.42 glyoxylate plus oxaloacetate Bacillus subtilis
1.1.1.42 glyoxylate plus oxaloacetate Chlamydomonas reinhardtii
1.1.1.42 glyoxylate plus oxaloacetate Thermus aquaticus
1.1.1.42 Oxalomalate
-
 Chlamydomonas reinhardtii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.42 0.0091
-
 D,L-isocitrate
-
 Bacillus subtilis
1.1.1.42 0.0097
-
 D,L-isocitrate 25°C assay temperature Thermus aquaticus
1.1.1.42 0.01
-
 NADP+
-
 Chlamydomonas reinhardtii
1.1.1.42 0.0125
-
 D,L-isocitrate
-
 Chlamydomonas reinhardtii
1.1.1.42 0.0165
-
 NADP+ 25°C assay temperature Thermus aquaticus
1.1.1.42 0.0181
-
 D,L-isocitrate 70°C assay temperature Thermus aquaticus
1.1.1.42 0.0274
-
 NADP+
-
 Bacillus subtilis
1.1.1.42 0.031
-
 NADP+ 70°C assay temperature Thermus aquaticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.42 60000 70000 gel filtration Thermus aquaticus
1.1.1.42 75000 80000 gel filtration Bacillus subtilis
1.1.1.42 80000
-
 gel filtration Escherichia coli
1.1.1.42 80000
-
 gel filtration Azotobacter vinelandii
1.1.1.42 87000 92600 gel filtration Geobacillus stearothermophilus
1.1.1.42 90000 100000 gel filtration Chlamydomonas reinhardtii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.42 isocitrate + NADP+ Thermus aquaticus
-
 2-oxoglutarate + NADPH + H+ + CO2
-
 ?
1.1.1.42 isocitrate + NADP+ Chlamydomonas reinhardtii
-
 2-oxoglutarate + NADPH + H+ + CO2
-
 ?
1.1.1.42 isocitrate + NADP+ Bacillus subtilis
-
 2-oxoglutarate + NADPH + H+ + CO2
-
 ?
1.1.1.42 isocitrate + NADP+ Escherichia coli
-
 2-oxoglutarate + NADPH + H+ + CO2
-
 ?
1.1.1.42 isocitrate + NADP+ Geobacillus stearothermophilus
-
 2-oxoglutarate + NADPH + H+ + CO2
-
 ?
1.1.1.42 isocitrate + NADP+ Azotobacter vinelandii
-
 2-oxoglutarate + NADPH + H+ + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.42 Azotobacter vinelandii
-
-
-
1.1.1.42 Bacillus subtilis
-
-
-
1.1.1.42 Chlamydomonas reinhardtii
-
-
-
1.1.1.42 Escherichia coli
-
-
-
1.1.1.42 Geobacillus stearothermophilus
-
-
-
1.1.1.42 Thermus aquaticus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.42
-
 Thermus aquaticus
1.1.1.42
-
 Chlamydomonas reinhardtii
1.1.1.42
-
 Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.42 isocitrate + NADP+
-
 Thermus aquaticus 2-oxoglutarate + NADPH + H+ + CO2
-
 ?
1.1.1.42 isocitrate + NADP+
-
 Chlamydomonas reinhardtii 2-oxoglutarate + NADPH + H+ + CO2
-
 ?
1.1.1.42 isocitrate + NADP+
-
 Bacillus subtilis 2-oxoglutarate + NADPH + H+ + CO2
-
 ?
1.1.1.42 isocitrate + NADP+
-
 Escherichia coli 2-oxoglutarate + NADPH + H+ + CO2
-
 ?
1.1.1.42 isocitrate + NADP+
-
 Geobacillus stearothermophilus 2-oxoglutarate + NADPH + H+ + CO2
-
 ?
1.1.1.42 isocitrate + NADP+
-
 Azotobacter vinelandii 2-oxoglutarate + NADPH + H+ + CO2
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.42 25
-
 assay condition Thermus aquaticus
1.1.1.42 25
-
 assay condition Chlamydomonas reinhardtii
1.1.1.42 25
-
 assay condition Bacillus subtilis
1.1.1.42 25
-
 assay condition Geobacillus stearothermophilus
1.1.1.42 37
-
-
 Bacillus subtilis
1.1.1.42 70
-
-
 Thermus aquaticus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.42 30
-
 50% loss of activity in 1 h Chlamydomonas reinhardtii
1.1.1.42 54
-
 50% loss of activity in 1 h Bacillus subtilis
1.1.1.42 88
-
 50% loss of activity in 1 h Thermus aquaticus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.42 6 9
-
 Thermus aquaticus