EC Number | General Stability | Organism |
---|---|---|
1.1.1.40 | malic acid decreases heat stability at pH values above 5.0 | Pigeon |
1.1.1.40 | Mg2+ or Mn2+ increase heat stability | Pigeon |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.40 | adenosine 2'-phosphate | - |
Pigeon | |
1.1.1.40 | ADP | - |
Pigeon | |
1.1.1.40 | arsenite | weak | Pigeon | |
1.1.1.40 | Cu2+ | 3 mM, weak | Pigeon | |
1.1.1.40 | diphenyliodonium chloride | weak | Pigeon | |
1.1.1.40 | Hg2+ | 0.0005 mM, almost complete inhibition | Pigeon | |
1.1.1.40 | iodoacetate | weak | Pigeon | |
1.1.1.40 | NAD+ | weak | Pigeon | |
1.1.1.40 | o-Iodosobenzoate | strong | Pigeon | |
1.1.1.40 | p-mercuribenzoate | strong | Pigeon |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.40 | 0.016 | - |
NADP+ | pH 7.5 | Pigeon | |
1.1.1.40 | 0.041 | - |
L-malate | pH 6.5 | Pigeon | |
1.1.1.40 | 0.39 | - |
L-malate | pH 7.5 | Pigeon | |
1.1.1.40 | 3.3 | - |
L-malate | pH 8.5 | Pigeon |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.40 | Co2+ | less effective activation than Mn2+ | Pigeon | |
1.1.1.40 | Cu2+ | weak activation | Pigeon | |
1.1.1.40 | Mg2+ | promotes activity | Pigeon | |
1.1.1.40 | Mn2+ | 0.004 mM, 50% of the maximal activity of the malic activity. 0.2 mM, 50% of the maximal activity of oxaloacetic decarboxylase | Pigeon | |
1.1.1.40 | Ni2+ | weak activation | Pigeon | |
1.1.1.40 | Zn2+ | weak activation | Pigeon |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.40 | Pigeon | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.40 | - |
Pigeon |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.1.40 | liver | - |
Pigeon | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.40 | additional information | - |
- |
Pigeon |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.40 | (S)-malate + NADP+ | the singly-ionized species is the substrate, doubly-ionized malate is unreactive | Pigeon | pyruvate + CO2 + NADPH | - |
r | |
1.1.1.40 | Oxaloacetate | - |
Pigeon | Pyruvate + CO2 | - |
? | |
1.1.1.40 | pyruvate + CO2 + NADPH | - |
Pigeon | L-malate + NADP+ | - |
r |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.40 | additional information | - |
Mg2+ or Mn2+ increase heat stability | Pigeon |
1.1.1.40 | additional information | - |
malic acid decreases heat stability at pH values above 5.0 | Pigeon |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.40 | 7.2 | - |
in presence of 0.1 mM malate | Pigeon |
1.1.1.40 | 8.5 | 9 | in presence of 0.1 M malate | Pigeon |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.40 | NADP+ | cofactor | Pigeon | |
1.1.1.40 | NADP+ | stimulation of decarboxylation of oxaloacetate | Pigeon | |
1.1.1.40 | NADPH | cofactor | Pigeon |