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Literature summary extracted from

  • Lin, R.C.; Davis, E.J.
    Malic enzymes of rabbit heart mitochondria. Separation and comparison of some characteristics of a nicotinamide adenine dinucleotide-preferring and a nicotinamide adenine dinuclweotide phosphate-specific enzyme (1974), J. Biol. Chem., 249, 3867-3875.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.39 ATP competitive with respect to (S)-malate Oryctolagus cuniculus
1.1.1.39 oxalate
-
 Oryctolagus cuniculus
1.1.1.40 oxalate
-
 Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.39 0.1
-
 NAD+ activation by Mn2+ Oryctolagus cuniculus
1.1.1.39 0.82
-
 NAD+ activation by Mg2+ Oryctolagus cuniculus
1.1.1.39 1.32
-
 NADP+ activation by Mn2+ Oryctolagus cuniculus
1.1.1.39 3.2
-
 (S)-malate with NAD+ as coenzyme, activation by Mn2+ Oryctolagus cuniculus
1.1.1.39 6.12
-
 NADP+ activation by Mg2+ Oryctolagus cuniculus
1.1.1.39 9.8
-
 (S)-malate with NADP+ as coenzyme, activation by Mn2+ Oryctolagus cuniculus
1.1.1.39 13
-
 (S)-malate with NAD+ as coenzyme, activation by Mg2+ Oryctolagus cuniculus
1.1.1.39 22.5
-
 (S)-malate with NADP+ as coenzyme, activation by Mg2+ Oryctolagus cuniculus
1.1.1.40 0.014
-
 NADP+ activation by Mn2+ Oryctolagus cuniculus
1.1.1.40 0.025
-
 NADP+ activation by Mg2+ Oryctolagus cuniculus
1.1.1.40 1.06
-
 (S)-malate activation by Mn2+ Oryctolagus cuniculus
1.1.1.40 1.06
-
 (S)-malate in the presence of 10 mM Mn2+, at pH 7.4 and 25°C Oryctolagus cuniculus
1.1.1.40 3.63
-
 (S)-malate activation by Mg2+ Oryctolagus cuniculus
1.1.1.40 3.63
-
 (S)-malate in the presence of 10 mM Mg2+, at pH 7.4 and 25°C Oryctolagus cuniculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.39 mitochondrion
-
 Oryctolagus cuniculus 5739
-
1.1.1.40 mitochondrion
-
 Oryctolagus cuniculus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.39 Mg2+ Mn2+ or Mg2+ required Oryctolagus cuniculus
1.1.1.39 Mg2+ Km with NAD+: 4.25 mM, Km with NADP+: 13.3 mM Oryctolagus cuniculus
1.1.1.39 Mn2+ Mn2+ or Mg2+ required Oryctolagus cuniculus
1.1.1.39 Mn2+ Km with NAD+: 0.14 mM, Km with NADP+: 0.81 mM Oryctolagus cuniculus
1.1.1.40 Mg2+ requires low concentration of Mn2+ or Mg2+ for activity, Km: 0.24 mM Oryctolagus cuniculus
1.1.1.40 Mn2+ Km: 0.01 mM Oryctolagus cuniculus
1.1.1.40 Mn2+ requires low concentration of Mn2+ or Mg2+ for activity Oryctolagus cuniculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.39 270000
-
 gel filtration Oryctolagus cuniculus
1.1.1.40 180000
-
 gel filtration Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.39 Oryctolagus cuniculus
-
-
-
1.1.1.40 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.39
-
 Oryctolagus cuniculus
1.1.1.40
-
 Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.39 heart
-
 Oryctolagus cuniculus
-
1.1.1.40 cardiac muscle
-
 Oryctolagus cuniculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.39 (S)-malate + NADP+
-
 Oryctolagus cuniculus pyruvate + CO2 + NADPH
-
 ir
1.1.1.39 malate + NAD+
-
 Oryctolagus cuniculus pyruvate + CO2 + NADH + H+
-
 ir
1.1.1.39 additional information no decarboxylation of oxaloacetate Oryctolagus cuniculus ?
-
 ?
1.1.1.40 (S)-malate + NADP+
-
 Oryctolagus cuniculus pyruvate + CO2 + NADPH
-
 r
1.1.1.40 Oxaloacetate
-
 Oryctolagus cuniculus Pyruvate + CO2
-
 ?
1.1.1.40 pyruvate + CO2 + NADPH 6% of the activity of the NADP+-linked oxidative decarboxylation of malate Oryctolagus cuniculus L-malate + NADP+
-
 r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.39 7
-
-
 Oryctolagus cuniculus
1.1.1.40 7 8
-
 Oryctolagus cuniculus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.39 NAD+ cofactor Oryctolagus cuniculus
1.1.1.39 NADP+
-
 Oryctolagus cuniculus