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Literature summary extracted from

  • Cuevas, I.C.; Podesta, F.E.
    Purification and physical and kinetic characterization of an NAD+-dependent malate dehydrogenase from leaves of pineapple (Ananas comosus) (2000), Physiol. Plant., 108, 240-248.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.37
-
 Mesembryanthemum crystallinum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.37 0.0152
-
 NADH pH 7.5 Ananas comosus
1.1.1.37 0.041
-
 NADH pH 6.8 Ananas comosus
1.1.1.37 0.0643
-
 oxaloacetate pH 6.8 Ananas comosus
1.1.1.37 0.22
-
 NAD+ pH 7.5 Ananas comosus
1.1.1.37 0.28
-
 NAD+ pH 6.8 Ananas comosus
1.1.1.37 0.287
-
 oxaloacetate pH 7.5 Ananas comosus
1.1.1.37 0.46
-
 L-malate pH 7.5 Ananas comosus
1.1.1.37 2.1
-
 L-malate pH 6.8 Ananas comosus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.37 chloroplast
-
 Zea mays 9507
-
1.1.1.37 chloroplast
-
 Arabidopsis thaliana 9507
-
1.1.1.37 cytoplasm
-
 Echinococcus granulosus 5737
-
1.1.1.37 cytosol
-
 Arabidopsis thaliana 5829
-
1.1.1.37 cytosol
-
 Echinococcus granulosus 5829
-
1.1.1.37 cytosol
-
 Mesembryanthemum crystallinum 5829
-
1.1.1.37 cytosol
-
 Ananas comosus 5829
-
1.1.1.37 cytosol
-
 Bryophyllum calycinum 5829
-
1.1.1.37 glyoxysome
-
 Ananas comosus 9514
-
1.1.1.37 glyoxysome
-
 Citrullus lanatus 9514
-
1.1.1.37 mitochondrion
-
 Arabidopsis thaliana 5739
-
1.1.1.37 mitochondrion
-
 Ananas comosus 5739
-
1.1.1.37 mitochondrion
-
 Citrullus lanatus 5739
-
1.1.1.37 mitochondrion
-
 Bryophyllum calycinum 5739
-
1.1.1.37 peroxisome
-
 Ananas comosus 5777
-
1.1.1.37 peroxisome
-
 Bryophyllum calycinum 5777
-
1.1.1.37 plastid
-
 Arabidopsis thaliana 9536
-
1.1.1.37 plastid
-
 Opuntia ficus-indica 9536
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.37 39500
-
 2 * 39500, pH 7.0, SDS-PAGE Ananas comosus
1.1.1.37 39500
-
 4 * 39500, pH 8.0, SDS-PAGE Ananas comosus
1.1.1.37 160000
-
 native PAGE Ananas comosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.37 malate + NAD+ Zea mays
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Arabidopsis thaliana
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Echinococcus granulosus
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Mesembryanthemum crystallinum
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Ananas comosus
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Citrullus lanatus
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Opuntia ficus-indica
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Bryophyllum calycinum
-
 oxaloacetate + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.37 Ananas comosus
-
 pineapple, cv. Havaiano
-
1.1.1.37 Arabidopsis thaliana
-
-
-
1.1.1.37 Bryophyllum calycinum
-
-
-
1.1.1.37 Citrullus lanatus
-
 watermelon
-
1.1.1.37 Echinococcus granulosus
-
-
-
1.1.1.37 Mesembryanthemum crystallinum
-
-
-
1.1.1.37 Opuntia ficus-indica
-
 cactus
-
1.1.1.37 Zea mays
-
 corn
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.37
-
 Ananas comosus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.37 cotyledon
-
 Citrullus lanatus
-
1.1.1.37 leaf
-
 Arabidopsis thaliana
-
1.1.1.37 leaf
-
 Ananas comosus
-
1.1.1.37 plant
-
 Ananas comosus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.37 1494
-
 cytoplasmic isoenzyme Ananas comosus
1.1.1.37 2000 2500 mitochondrial isoenzyme Ananas comosus

Storage Stability

EC Number Storage Stability Organism
1.1.1.37 -20°C, activity tends to decay rapidly after purification Ananas comosus
1.1.1.37 -80°C, remains active for several weeks Ananas comosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.37 (S)-malate + NAD+
-
 Zea mays oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Arabidopsis thaliana oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Echinococcus granulosus oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Mesembryanthemum crystallinum oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Ananas comosus oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Citrullus lanatus oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Opuntia ficus-indica oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Bryophyllum calycinum oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Zea mays oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Arabidopsis thaliana oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Echinococcus granulosus oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Mesembryanthemum crystallinum oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Ananas comosus oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Citrullus lanatus oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Opuntia ficus-indica oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Bryophyllum calycinum oxaloacetate + NADH + H+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Zea mays L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Arabidopsis thaliana L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Echinococcus granulosus L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Mesembryanthemum crystallinum L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Ananas comosus L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Citrullus lanatus L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Opuntia ficus-indica L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Bryophyllum calycinum L-malate + NAD+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.37 dimer 2 * 39500, pH 7.0, SDS-PAGE Ananas comosus
1.1.1.37 tetramer 4 * 39500, pH 8.0, SDS-PAGE Ananas comosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.37 80
-
 activity increases up to Ananas comosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.37 6.6 7 oxaloacetate reduction Ananas comosus
1.1.1.37 6.8 7
-
 Ananas comosus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.37 NAD+ no detectable activity with NADP+ Ananas comosus