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Literature summary extracted from

  • Drmota, T.; Tachezy, J.; Kulda, J.
    Isolation and characterization of cytosolic malate dehydrogenase from Trichomonas vaginalis (1997), Folia Parasitol., 44, 103-108.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.37 medicine therapy development for sexually transmitted disease of humans Trichomonas vaginalis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.37 oxaloacetate
-
 Trichomonas gallinae
1.1.1.37 oxaloacetate
-
 Trichomonas vaginalis
1.1.1.37 oxaloacetate
-
 Tritrichomonas suis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.37 0.0106
-
 NADH Tv 10-02 Trichomonas vaginalis
1.1.1.37 0.011
-
 NADH
-
 Trichomonas gallinae
1.1.1.37 0.013
-
 NADH
-
 Tritrichomonas suis
1.1.1.37 0.016
-
 oxaloacetate
-
 Trichomonas gallinae
1.1.1.37 0.0162
-
 oxaloacetate Tv 10-02 Trichomonas vaginalis
1.1.1.37 0.0175
-
 oxaloacetate
-
 Tritrichomonas suis
1.1.1.37 0.02
-
 NADH Tv-1961 Trichomonas vaginalis
1.1.1.37 0.02
-
 oxaloacetate Tv-1961 Trichomonas vaginalis
1.1.1.37 0.02
-
 NADH Tv-1973 Trichomonas vaginalis
1.1.1.37 0.022
-
 NAD+ Tv-1973 Trichomonas vaginalis
1.1.1.37 0.043
-
 oxaloacetate Tv-1973 Trichomonas vaginalis
1.1.1.37 0.0699
-
 NAD+ Tv 10-02 Trichomonas vaginalis
1.1.1.37 0.07
-
 NAD+
-
 Trichomonas gallinae
1.1.1.37 0.2
-
 L-malate
-
 Trichomonas gallinae
1.1.1.37 1
-
 L-malate Tv-1961 Trichomonas vaginalis
1.1.1.37 1.22
-
 L-malate Tv 10-02 Trichomonas vaginalis
1.1.1.37 1.4
-
 L-malate Tv-1973 Trichomonas vaginalis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.37 cytosol
-
 Trichomonas vaginalis 5829
-
1.1.1.37 hydrogenosome
-
 Trichomonas vaginalis 42566
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.37 34500
-
 2 * 34500, SDS-PAGE Trichomonas vaginalis
1.1.1.37 37000
-
 6 * 37000 Tritrichomonas suis
1.1.1.37 60000
-
 gel filtration Trichomonas vaginalis
1.1.1.37 72000
-
 gel filtration Trichomonas gallinae
1.1.1.37 244000
-
 gel filtration Tritrichomonas suis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.37 malate + NAD+ Thermus aquaticus
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Trichomonas vaginalis
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Mycobacterium leprae
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Tritrichomonas suis
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Trichomonas gallinae
-
 oxaloacetate + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.37 Mycobacterium leprae
-
-
-
1.1.1.37 Thermus aquaticus
-
-
-
1.1.1.37 Trichomonas gallinae
-
-
-
1.1.1.37 Trichomonas vaginalis
-
 strain TV 10-02, strain Tv-1961, strain Tv-1973
-
1.1.1.37 Tritrichomonas suis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.37
-
 Trichomonas vaginalis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.37 352.7
-
-
 Trichomonas vaginalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.37 (S)-malate + NAD+
-
 Thermus aquaticus oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Trichomonas vaginalis oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Mycobacterium leprae oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Tritrichomonas suis oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Trichomonas gallinae oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Thermus aquaticus oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Trichomonas vaginalis oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Mycobacterium leprae oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Tritrichomonas suis oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Trichomonas gallinae oxaloacetate + NADH + H+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Thermus aquaticus L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Trichomonas vaginalis L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Mycobacterium leprae L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Tritrichomonas suis L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Trichomonas gallinae L-malate + NAD+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.37 dimer
-
 Thermus aquaticus
1.1.1.37 dimer 2 * 34500, SDS-PAGE Trichomonas vaginalis
1.1.1.37 hexamer 6 * 37000 Tritrichomonas suis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.37 7.5 8.3 Tv 10-02, reduction of oxaloacetate Trichomonas vaginalis
1.1.1.37 7.8
-
 strain Tv-1973 Trichomonas vaginalis
1.1.1.37 7.8 7.9 Tv-1961, reduction of oxaloacetate Trichomonas vaginalis
1.1.1.37 8
-
 oxaloacetate reduction Tritrichomonas suis
1.1.1.37 9
-
 oxalacetate reduction Trichomonas gallinae
1.1.1.37 9
-
 malate oxidation, above pH 9.0, strain Tv-1973 Trichomonas vaginalis
1.1.1.37 9.2 10.7 malate oxidation, strain Tv-1961 Trichomonas vaginalis
1.1.1.37 9.5
-
 reverse reaction from malate to oxalacetate Trichomonas vaginalis