Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Grossebueter, W.; Hartl, T.; Goerisch, H.; Stezowski, J.J.
    Purification and properties of malate dehydrogenase from the thermophilic archaebacterium Thermoplasma acidophilum (1986), Biol. Chem. Hoppe-Seyler, 367, 457-463.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.37
-
 Thermoplasma acidophilum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.37 0.0056
-
 oxaloacetate
-
 Thermoplasma acidophilum
1.1.1.37 0.0117
-
 NADH
-
 Thermoplasma acidophilum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.37 36600
-
 4 * 36600, SDS-PAGE Thermoplasma acidophilum
1.1.1.37 105000
-
 gel filtration Thermoplasma acidophilum
1.1.1.37 130000
-
 sedimentation equilibrium experiment Thermoplasma acidophilum
1.1.1.37 144000
-
 cross-linking data Thermoplasma acidophilum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.37 malate + NAD+ Bacillus subtilis
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Halobacterium salinarum
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Sulfolobus acidocaldarius
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Thermoplasma acidophilum
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Bacillus sp. (in: Bacteria)
-
 oxaloacetate + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.37 Bacillus sp. (in: Bacteria)
-
-
-
1.1.1.37 Bacillus subtilis
-
-
-
1.1.1.37 Halobacterium salinarum
-
-
-
1.1.1.37 Methanospirillum hungatei
-
-
-
1.1.1.37 Sulfolobus acidocaldarius
-
-
-
1.1.1.37 Thermoplasma acidophilum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.37
-
 Thermoplasma acidophilum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.37 180
-
-
 Thermoplasma acidophilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.37 (S)-malate + NAD+
-
 Bacillus subtilis oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Halobacterium salinarum oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Sulfolobus acidocaldarius oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Thermoplasma acidophilum oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Bacillus sp. (in: Bacteria) oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Bacillus subtilis oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Halobacterium salinarum oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Sulfolobus acidocaldarius oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Thermoplasma acidophilum oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Bacillus sp. (in: Bacteria) oxaloacetate + NADH + H+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Bacillus subtilis L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Halobacterium salinarum L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Sulfolobus acidocaldarius L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Thermoplasma acidophilum L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Bacillus sp. (in: Bacteria) L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Methanospirillum hungatei L-malate + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.37 tetramer
-
 Bacillus subtilis
1.1.1.37 tetramer
-
 Sulfolobus acidocaldarius
1.1.1.37 tetramer
-
 Bacillus sp. (in: Bacteria)
1.1.1.37 tetramer 4 * 36600, SDS-PAGE Thermoplasma acidophilum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.37 65
-
-
 Thermoplasma acidophilum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.37 60
-
 quite heat-stable, stable up to, loses 50% of its original activity after 10 min at 77°C Thermoplasma acidophilum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.37 1.8
-
 oxaloacetate
-
 Thermoplasma acidophilum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.37 8.1
-
 reduction of oxaloacetate Thermoplasma acidophilum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.37 NAD+
-
 Sulfolobus acidocaldarius
1.1.1.37 NAD+ purified enzyme shows activity with both coenzymes Thermoplasma acidophilum
1.1.1.37 NADP+
-
 Sulfolobus acidocaldarius
1.1.1.37 NADP+ purified enzyme shows activity with both coenzymes Thermoplasma acidophilum